Shim-pack Scepter C18-300
Shimadzu Shim-pack Scepter C18-300 Analytical Column, 1.9μm, 300Å, 3mm x 150mm, 1/pk
Part Number: 227-31203-10
Hybrid Technology for Versatility and Superior Performance
Broad pH tolerance (pH 1 to 12*) for use with a wide range of method conditions Eight stationary phases and Three column hardware types for extensive sample coverage Scalability from analytical UHPLC to preparative purification applications
Achieve excellent stability and performance over a wide range of LC conditions with Shim-pack Scepter™- the next generation organic silica hydrid-based LC columns. Featuring different ligand functional groups, Shim-pack Scepter columns are effective for method development/scouting, and are suitable for a wide variety of applications and sample types. The three particle sizes (1.9 µm, 3 µm, 5 µm) and wide range of column dimensions make Shim-pack Scepter LC columns fully scalable between UHPLC, HPLC, and preparative LC for seamless method transfer between different laboratory instrumentation.
Fully Porous Hybrid Particle Based Column Series
Excellent stability and performance could be achieved under a wide range of LC conditions with Shim-pack Scepter™ LC columns, which are the next generation organic silica hybrid based columns. With different chemistry characteristics, Shim-pack Scepter columns are effective for method development/scouting with suitability for use in a wide variety of applications.
With different particle sizes (1.9 μm, 3 μm, 5 μm) and different column dimensions, Shim-pack Scepter LC columns are fully scalable between UHPLC, HPLC and preparative LC making method transfer seamless between different laboratory instrumentation.
Shim-pack Scepter C18-300
Shim-pack Scepter C18-300 is a wide-pore organic silica hybrid reversed phase column recommended for the separation of proteins such as monoclonal antibodies and mid-sized molecules such as oligonucleic acids and peptides that may not be retained on 100-120Å pore size columns due to size-exclusion effects. Pore sizes are optimized for large molecular weight compounds and dispersed uniformly in the column, resulting in symmetrical peak shapes and increased resolution of antibodies and nucleic acids compared to a smaller pore size column.
Shim-pack Scepter Chemistries
Shim-pack Scepter | Reversed Phase | ||||
C18-120 | C18-300 | HD-C18-80 | C8-120 | C4-300 | |
Ligand Type | Trifunctional C18 | Trifunctional C18 | Trifunctional C18 | Trifunctional C8 | Trifunctional C4 |
General Purpose | General Purpose for Large Molecules | High Density Type for Increased Retention | |||
Particle | Organic Silica Hybrid | ||||
Particle Size | 1.9 µm, 3 µm, 5 µm | ||||
Pore Size | 12 nm (120 Å) | 30 nm (300 Å) | 8 nm (80Å) | 12 nm (120 Å) | 30 nm (300 Å) |
End Capping | Proprietary | ||||
pH Range | 1 - 12 | 1 - 10 | |||
100% Aqueous Condition | YES | YES | x | x | YES |
USP Classification | L1 | L1 | L1 | L7 | L26 |
Shim-pack Scepter | Reversed Phase | HILIC | |
Phenyl | PFPP | Diol-H | |
Ligand Type | Trifunctional Phenylbutyl | Trifunctional Pentafluorophenylpropyl | Trifunctional Dihydroxypropyl |
Particle | Organic Silica Hybrid | ||
Particle Size | 1.9 µm, 3 µm, 5 µm | ||
Pore Size | 12 nm (120Å) | ||
End Capping | Proprietary | None | |
pH Range | 1 - 10 | 1 - 8 | 2 - 10 |
100% Aqueous Condition | YES | YES | - |
USP Classification | L11 | L43 | L20 |
Specifications:
Shimadzu Product Name: | Shim-pack Scepter C18-300, 1.9um, 3.0x150 |
Product Type: | High-performance liquid chromatography (HPLC) Column |
Column Name: | Scepter C18-300 |
Particle size: | 1.9 µm |
ID: | 3 mm |
Length: | 150 mm |
USP: | L1 |
Functional Group: | C18 |
Mode: | Reversed Phase |
Column Type: | Analytical column |
Guard Column: | 227-31206-02 |
Guard Column Holder: | 227-32041-01 |
Pore size: | 30 nm / 300 Å |
End Capping: | Yes |
PH: | 1 - 12 |
Pressure Tolerance: | 100 MPa |
Pack size: | 1 |
- Model: 227-31203-10
- Manufactured by: Shimadzu