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Shimadzu Shim-pack Scepter C4-300 Claris Bioinert Column, 3 µm, 300 Å, 4.6 x 100 mm, 1/Pk - 227-31208-08

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Shim-pack Scepter C4-300

Shimadzu Shim-pack Scepter C4-300 Claris Bioinert Analytical Column, 3µm, 300Å, 4.6mm x 100mm, 1/Pk

Part Number: 227-31208-08

The Scepter Claris column has a bioinert surface treatment on the column body and frit for a completely metal-free flowpath. It is ideal for the analysis of compounds that adsorb to either stainless steel or PEEK. The C4-300 phase is recommended for mid- to large-sized molecules (above 5000 MW) to ensure retention and avoid size exclusion effects. It is ideal for LC and LCMS analysis of peptides, proteins, intact monoclonal antibodies and their subunits up to 150 kDa. Bioinert coating eliminates adsorption and reduces tailing for compounds that have strong interactions with stainless steel or PEEK surfaces. USP L26, pore size: 300A, pH range: 1-10, max pressure: 45 MPa/4500 bar/6,500 psi, fully endcapped. Similar to XBridge Premier Protein BEH C4.

Shim-pack Scepter Claris features a column body with a newly-developed bioinert coating packed with Scepter series stationary phases.

  • Bioinert coating is applied to the column body and stainless steel frit
  • Ideal for analysis of metal-coordinating and hydrophobically adsorbing compounds such as nucleic acids, proteins, and lipids
  • Outstanding pH and lifetime stability due to Scepter organic silica hybrid packing

Shim-pack Scepter C4-300

Shim-pack Scepter C4-300 is wide-pore organic silica hybrid reversed phase columns recommended for the separation of proteins such as monoclonal antibodies and mid-sized molecules such as oligonucleic acids and peptides that may not be retained on 100-120Å pore size columns due to size-exclusion effects. The organic silica hybrid base material is highly stable even at high temperatures under acidic and basic mobile phase conditions. Pore sizes are optimized for large molecular weight compounds and dispersed uniformly in the column, resulting in symmetrical peak shapes and increased resolution of antibodies and nucleic acids compared to a smaller pore size column. Shim-pack Scepter C4-300 is also effective for high-sensitivity LC/MS analysis, producing good peak shapes even under weak ionpairing conditions using a formic acid mobile phase.

Hybrid Technology for Versatility and Superior Performance

Broad pH tolerance (pH 1 to 12*) for use with a wide range of method conditions Eight stationary phases and Three column hardware types for extensive sample coverage Scalability from analytical UHPLC to preparative purification applications

Achieve excellent stability and performance over a wide range of LC conditions with Shim-pack Scepter™- the next generation organic silica hydrid-based LC columns. Featuring different ligand functional groups, Shim-pack Scepter columns are effective for method development/scouting, and are suitable for a wide variety of applications and sample types. The three particle sizes (1.9 µm, 3 µm, 5 µm) and wide range of column dimensions make Shim-pack Scepter LC columns fully scalable between UHPLC, HPLC, and preparative LC for seamless method transfer between different laboratory instrumentation.

Fully Porous Hybrid Particle Based Column Series

Excellent stability and performance could be achieved under a wide range of LC conditions with Shim-pack Scepter™ LC columns, which are the next generation organic silica hybrid based columns. With different chemistry characteristics, Shim-pack Scepter columns are effective for method development/scouting with suitability for use in a wide variety of applications.

With different particle sizes (1.9 μm, 3 μm, 5 μm) and different column dimensions, Shim-pack Scepter LC columns are fully scalable between UHPLC, HPLC and preparative LC making method transfer seamless between different laboratory instrumentation.

Shim-pack Scepter Chemistries

Shim-pack ScepterReversed Phase
C18-120C18-300HD-C18-80C8-120C4-300
Ligand TypeTrifunctional C18Trifunctional C18Trifunctional C18Trifunctional C8Trifunctional C4
General PurposeGeneral Purpose for Large MoleculesHigh Density Type for Increased Retention
ParticleOrganic Silica Hybrid
Particle Size1.9 µm, 3 µm, 5 µm
Pore Size12 nm (120 Å)30 nm (300 Å)8 nm (80Å)12 nm (120 Å)30 nm (300 Å)
End CappingProprietary
pH Range1 - 121 - 10
100% Aqueous ConditionYESYESxxYES
USP ClassicationL1L1L1L7L26

 

Shim-pack ScepterReversed PhaseHILIC
PhenylPFPPDiol-H
Ligand TypeTrifunctional PhenylbutylTrifunctional PentafluorophenylpropylTrifunctional Dihydroxypropyl
ParticleOrganic Silica Hybrid
Particle Size1.9 µm, 3 µm, 5 µm
Pore Size12 nm (120Å)
End CappingProprietaryNone
pH Range1 - 101 - 82 - 10
100% Aqueous ConditionYESYES-
USP ClassificationL11L43L20

 

Specifications:

Shimadzu Product DescriptionShim-pack Scepter Claris C4-300, 3um, 4.6 x 100mm LC Column
Column TypeAnalytical column (Bioinert)
Line UpShim-pack
SeriesScepter
Column NameScepter Claris C4-300
ModificationC4
For use in
  • High-performance liquid chromatography (HPLC)
  • Ultra-High Performance Liquid Chromatography (UHPLC) 
  • Liquid chromatography–mass spectrometry (LC–MS)
  • Lliquid chromatography (LC)
Particle size3 µm
Inner Diameter4.6 mm
Length100 mm
Pore size30 nm / 300 Å
End CappingYes
Column ModeReversed phase
USPL26
Pressure tolerance45 MPa
Content1 pc

  • Model: 227-31208-08
  • Manufactured by: Shimadzu