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Shimadzu Shim-pack Scepter C4-300 Analytical Column, 5 µm, 300 Å, 3.0 x 75 mm, 1/Pk - 227-31181-02

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Shim-pack Scepter C4-300 

Shimadzu Shim-pack Scepter C4-300 Analytical Column, 5µm, 300Å, 3mm x 75mm, 1/Pk

Part Number: 227-31181-02

Shim-pack Scepter LC column series products provide excellent durability and performance under a wide range of conditions because an organic silica hybrid base is used. With a wide range of particle sizes and column sizes, the series is fully compatible with UHPLC through preparative HPLC. The Shim-pack Scepter 300-C4 is the most suitable reversed-phase column for LC as well as LC-MS analysis of proteins such as intact monoclonal antibodies and their sub-units. The Shim-pack Scepter LC column series products use an organic silica hybrid base, provide excellent durability and performance under a wide range of conditions. Due to a lineup of a wide variety of column chemistries with different separation selectivity (6 types of reverse phase, 1 type of HILIC), the columns are effective for method development/scouting and users can select a column that is suitable for each application. Furthermore, with a wide variety of particle sizes and column sizes, analysis method can be transferred seamlessly between UHPLC, analysis HPLC, and preparative HPLC. 

The Shim-pack Scepter 300-C4 is a wide-bore C4 column that uses an organic silica hybrid base which is suitable for separation of proteins such as monoclonal antibodies. The column can be used for analysis of proteins with a molecular weight of up to approximately 150,000 and is suitable for analysis of antibodies as it is very stable even under acidic or high-temperature conditions because an organic silica hybrid base is used. It is also effective for high-sensitivity analysis on LC/MS as it provides good peak shapes even when using a formic acid mobile phase with low ion paring effect. It is effective for separation of highly-hydrophobic middle and low-molecules that are hard to separate using a regular C18 column.

Hybrid Technology for Versatility and Superior Performance

Broad pH tolerance (pH 1 to 12*) for use with a wide range of method conditions Eight stationary phases and Three column hardware types for extensive sample coverage Scalability from analytical UHPLC to preparative purification applications

Achieve excellent stability and performance over a wide range of LC conditions with Shim-pack Scepter™- the next generation organic silica hydrid-based LC columns. Featuring different ligand functional groups, Shim-pack Scepter columns are effective for method development/scouting, and are suitable for a wide variety of applications and sample types. The three particle sizes (1.9 µm, 3 µm, 5 µm) and wide range of column dimensions make Shim-pack Scepter LC columns fully scalable between UHPLC, HPLC, and preparative LC for seamless method transfer between different laboratory instrumentation.

Fully Porous Hybrid Particle Based Column Series

Excellent stability and performance could be achieved under a wide range of LC conditions with Shim-pack Scepter™ LC columns, which are the next generation organic silica hybrid based columns. With different chemistry characteristics, Shim-pack Scepter columns are effective for method development/scouting with suitability for use in a wide variety of applications.

With different particle sizes (1.9 μm, 3 μm, 5 μm) and different column dimensions, Shim-pack Scepter LC columns are fully scalable between UHPLC, HPLC and preparative LC making method transfer seamless between different laboratory instrumentation.

Specifications:

Shimadzu Product Description Shim-pack Scepter C4-300, 5um, 3.0 x 75mm LC Column
Line Up Shim-pack
Series Scepter
Column Type Analytical column
Column Name Scepter C4-300
Modification C4
For use in
  • High-performance liquid chromatography (HPLC)
  • Ultra-High Performance Liquid Chromatography (UHPLC) 
  • Liquid chromatography–mass spectrometry (LC–MS)
  • Lliquid chromatography (LC)
Particle size 5 µm
Inner Diameter 3 mm
Length 75 mm
Pore size 30 nm / 300 Å
Guard Column:  227-31192-01
Guard Column Holder: 227-31172-03
End Capping Yes
Carbon Load 9%
Column Mode Reversed phase
pH range 1 - 10
USP L26
Pressure tolerance 45 Mpa

  • Model: 227-31181-02
  • Manufactured by: Shimadzu