Shimadzu Shim-pack Scepter C4-300 Preparative Column, 5 µm, 300 Å, 20 x 150 mm, 1/Pk - 227-31185-03

Shim-pack Scepter C4-300 

Shimadzu Shim-pack Scepter C4-300 Preparative Column, 5µm, 300Å, 20mm x 150mm, 1/Pk

Part Number: 227-31185-03

Shim-pack Scepter prep columns consist of an organosilane hybrid particle for excellent stability and performance and reliable scaleup up from a Scepter C4-300 analytical column The C4-300 phase is recommended for mid- to large-sized molecules (above 5000 MW) to ensure retention and avoid size exclusion effects. It is ideal for LC and LCMS analysis of peptides, proteins, intact monoclonal antibodies and their subunits up to 150 kDa, and compatible with mobile phase conditions up to pH 10 and 100% aqueous. USP L26, pore size: 300A, pH range: 1-10, max pressure: 45 MPa/450 bar/6,500 psi, fully endcapped. Method development on an analytical Scepter C4-300 column is highly recommended before purchasing a prep column. Prep columns cannot be returned or exchanged once used. Use with prep guard cartridges 227-31194-03 and holder 227-31171-03 to prolong the life of the column. Similar to XBridge Protein BEH C4.

The Shim-pack Scepter 300-C4 is a wide-bore C4 column that uses an organic silica hybrid base which is suitable for separation of proteins such as monoclonal antibodies. The column can be used for analysis of proteins with a molecular weight of up to approximately 150,000 and is suitable for analysis of antibodies as it is very stable even under acidic or high-temperature conditions because an organic silica hybrid base is used. It is also effective for high-sensitivity analysis on LC/MS as it provides good peak shapes even when using a formic acid mobile phase with low ion paring effect. It is effective for separation of highly-hydrophobic middle and low-molecules that are hard to separate using a regular C18 column.

Hybrid Technology for Versatility and Superior Performance

Broad pH tolerance (pH 1 to 12*) for use with a wide range of method conditions Eight stationary phases and Three column hardware types for extensive sample coverage Scalability from analytical UHPLC to preparative purification applications

Achieve excellent stability and performance over a wide range of LC conditions with Shim-pack Scepter™- the next generation organic silica hydrid-based LC columns. Featuring different ligand functional groups, Shim-pack Scepter columns are effective for method development/scouting, and are suitable for a wide variety of applications and sample types. The three particle sizes (1.9 µm, 3 µm, 5 µm) and wide range of column dimensions make Shim-pack Scepter LC columns fully scalable between UHPLC, HPLC, and preparative LC for seamless method transfer between different laboratory instrumentation.

Fully Porous Hybrid Particle Based Column Series

Excellent stability and performance could be achieved under a wide range of LC conditions with Shim-pack Scepter™ LC columns, which are the next generation organic silica hybrid based columns. With different chemistry characteristics, Shim-pack Scepter columns are effective for method development/scouting with suitability for use in a wide variety of applications.

With different particle sizes (1.9 μm, 3 μm, 5 μm) and different column dimensions, Shim-pack Scepter LC columns are fully scalable between UHPLC, HPLC and preparative LC making method transfer seamless between different laboratory instrumentation.

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