Shimadzu Shim-pack Scepter C4-300 Metal Free Column, 5 µm, 300 Å, 4.6 x 50 mm, 1/Pk - 227-31201-01

Shim-pack Scepter C4-300 Metal-Free

Shimadzu Shim-pack Scepter C4-300 Metal Free Analytical Column, 5µm, 300Å, 4.6mm x 50mm, 1/Pk

Part Number: 227-31201-01

The bioinert Scepter column has a PEEK-lined stainless steel body with a high pressure polymer frit for a completely metal-free flowpath. The C4-300 phase is recommended for mid- to large-sized molecules (above 5000 MW) to ensure retention and avoid size exclusion effects. It is ideal for LC and LCMS analysis of peptides, proteins, intact monoclonal antibodies and their subunits up to 150 kDa. PLS hardware eliminates adsorption and reduces tailing for compounds that tend to form chelates with metal surfaces, especially those containing phosphate groups. USP L26, pore size: 300A, pH range: 1-10, max pressure: 45 MPa/4500 bar/6,500 psi, fully endcapped. Phase is similar to XBridge Protein BEH C4, PLS hardware is unique.

Shim-pack Scepter (metal-free) is an inert column with a PEEK-lined stainless steel body designed for ultra-high-performance
analysis. All wetted surfaces including the column body and frit are metal-free.

• Ideal for analysis of metal-coordinating and ion-adsorbing compounds such as phosphate-containing and basic analytes
• Outstanding pH and lifetime stability due to Scepter organic silica hybrid packing

Shim-pack Scepter C4-300

Shim-pack Scepter C4-300 is wide-pore organic silica hybrid reversed phase columns recommended for the separation of proteins such as monoclonal antibodies and mid-sized molecules such as oligonucleic acids and peptides that may not be retained on 100-120Å pore size columns due to size-exclusion effects. The organic silica hybrid base material is highly stable even at high temperatures under acidic and basic mobile phase conditions. Pore sizes are optimized for large molecular weight compounds and dispersed uniformly in the column, resulting in symmetrical peak shapes and increased resolution of antibodies and nucleic acids compared to a smaller pore size column. Shim-pack Scepter C4-300 is also effective for high-sensitivity LC/MS analysis, producing good peak shapes even under weak ionpairing conditions using a formic acid mobile phase.

Hybrid Technology for Versatility and Superior Performance

Broad pH tolerance (pH 1 to 12*) for use with a wide range of method conditions Eight stationary phases and Three column hardware types for extensive sample coverage Scalability from analytical UHPLC to preparative purification applications

Achieve excellent stability and performance over a wide range of LC conditions with Shim-pack Scepter™- the next generation organic silica hydrid-based LC columns. Featuring different ligand functional groups, Shim-pack Scepter columns are effective for method development/scouting, and are suitable for a wide variety of applications and sample types. The three particle sizes (1.9 µm, 3 µm, 5 µm) and wide range of column dimensions make Shim-pack Scepter LC columns fully scalable between UHPLC, HPLC, and preparative LC for seamless method transfer between different laboratory instrumentation.

Fully Porous Hybrid Particle Based Column Series

Excellent stability and performance could be achieved under a wide range of LC conditions with Shim-pack Scepter™ LC columns, which are the next generation organic silica hybrid based columns. With different chemistry characteristics, Shim-pack Scepter columns are effective for method development/scouting with suitability for use in a wide variety of applications.

With different particle sizes (1.9 μm, 3 μm, 5 μm) and different column dimensions, Shim-pack Scepter LC columns are fully scalable between UHPLC, HPLC and preparative LC making method transfer seamless between different laboratory instrumentation.

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