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Shimadzu Shim-pack Scepter Diol-HILIC-120 Claris Bioinert Column, 5 µm, 120 Å, 4. m x 100 mm, 1/Pk - 227-31213-14

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Shim-pack Scepter™ Diol-HILIC-120 Claris

Shimadzu Shim-pack Scepter Diol-HILIC-120 Claris Bioinert Analytical Column, 5µm, 120Å, 4.6mm x 100mm, 1/Pk

Part Number: 227-31213-14

The Scepter Claris column has a bioinert surface treatment on the column body and frit for a completely metal-free flowpath. It is ideal for the analysis of compounds that adsorb to either stainless steel or PEEK. The organosilane hybrid particle with diol ligand is recommended for the analysis of polar and hydrophilic compounds that have limited or no retention on alkyl phases like C8 and C18. Ideal for LCMS use since ion-pairing reagents are not required, and the high organic mobile phases result in efficient solvent evaporation for increased sensitivity. Bioinert coating eliminates adsorption and reduces tailing for compounds that have strong interactions with stainless steel or PEEK surfaces. USP L20, pore size: 120A, pH range: 2-10, max pressure: 45 MPa/450 bar/6,500 psi, 12% carbon load, non-endcapped.

Shim-pack Scepter Claris features a column body with a newly-developed bioinert coating packed with Scepter series stationary phases.

  • Bioinert coating is applied to the column body and stainless steel frit
  • Ideal for analysis of metal-coordinating and hydrophobically adsorbing compounds such as nucleic acids, proteins, and lipids
  • Outstanding pH and lifetime stability due to Scepter organic silica hybrid packing

Shim-pack Scepter Series Chemistries

  Reveresed Phase HILIC
Chemistry C18-120 C18-300 HD-C18-80 C8-120 C4-300 Phenyl PFPP Diol-HILIC
Ligand Type Trifunctional C18 Trifunctional C18 Trifunctional C18 Trifunctional C8 Trifunctional C4 Trifunctional
Phenyl-butyl
Trifunctional
Pentafluorophenyl-propyl
Trifunctional
Dihydroxy-propyl
General Purpose General Purpose for
Large Molecules
High Density Type for
Increased Retention
Bonded Phase Octadecyl groups Octadecyl groups
(High Density type)
Octyl groups Butyl groups Phenylbutyl groups Pentaflorophenyl
propyl groups
Dihydroxypropyl
groups
Particle Organic Silica Hybrid
Particle Size 1.9 μm, 3 μm, 5 μm
Pore Size 12 nm (120Å) 30 nm (300Å) 8 nm (80Å) 12 nm 30 nm 12 nm (120Å)
End Capping Proprietary None
pH Range 1 - 12 1 - 10 1 - 8 2 - 10
100 % aqueous condition Yes Yes No No Yes Yes Yes N/A
USP Code L1 L1 L7 L26 L11 L43 L20

 

Hybrid Technology for Versatility and Superior Performance

Broad pH tolerance (pH 1 to 12*) for use with a wide range of method conditions Eight stationary phases and Three column hardware types for extensive sample coverage Scalability from analytical UHPLC to preparative purification applications

Achieve excellent stability and performance over a wide range of LC conditions with Shim-pack Scepter™- the next generation organic silica hydrid-based LC columns. Featuring different ligand functional groups, Shim-pack Scepter columns are effective for method development/scouting, and are suitable for a wide variety of applications and sample types. The three particle sizes (1.9 µm, 3 µm, 5 µm) and wide range of column dimensions make Shim-pack Scepter LC columns fully scalable between UHPLC, HPLC, and preparative LC for seamless method transfer between different laboratory instrumentation.

Fully Porous Hybrid Particle Based Column Series

Excellent stability and performance could be achieved under a wide range of LC conditions with Shim-pack Scepter™ LC columns, which are the next generation organic silica hybrid based columns. With different chemistry characteristics, Shim-pack Scepter columns are effective for method development/scouting with suitability for use in a wide variety of applications.

With different particle sizes (1.9 μm, 3 μm, 5 μm) and different column dimensions, Shim-pack Scepter LC columns are fully scalable between UHPLC, HPLC and preparative LC making method transfer seamless between different laboratory instrumentation.

Specifications:

Shimadzu Product Description Shim-pack Scepter Claris Diol-HILIC-120, 5um, 4.6 x 100mm LC Column
Line Up Shim-pack
Series Scepter
Column Type Analytical column (Bioinert)
Column Name Scepter Claris Diol-HILIC-120
Functional Group Diol-HILIC
Mode HILIC
For use in
  • High-performance liquid chromatography (HPLC)
  • Ultra-High Performance Liquid Chromatography (UHPLC) 
  • Liquid chromatography–mass spectrometry (LC–MS)
  • Lliquid chromatography (LC)
Particle size 5 µm
Inner Diameter 4.6 mm
Length 100 mm
Pore size 12 nm / 120 Å
End Capping No
Column Mode Reversed phase
pH range 2 - 10
USP L20
Pressure tolerance 45 Mpa

  • Model: 227-31213-14
  • Manufactured by: Shimadzu