Hydrophilic Interaction Chromatography
ULTRON AF-HILIC-CD HPLC Narrow Bore Column, 120 Å, 2 μm, 150 mm Length x 2.0 mm ID
Shinwa Chemical Industries Ltd. Part Number: LCC-13311-151
Previously AF-HILIC-CD (HT), this column employs a 2µm packing material. This column can be used for high-throughput analysis of structural homologues and structural isomers.
ULTRON AF-HILIC-CD series is a HILIC mode column in which β-cyclodextrin (?) is chemically bonded to a silica stationary phase via a spacer. The selectivity towards molecular structure is high, and also shows high resolution analysis for structural analogues and structural isomers of acidic, basic and neutral compounds.
ULTRON AF-HILIC-CD has a hallow cavity derived from its cyclic structure and are hydrophobic on the inside and hydrophilic on the outside. It is characterized by the inclusion complex formation that takes with various compounds by the intermolecular force inside the cyclic structure.
Hydrophilic Interaction Chromatography (HILIC) is a separation mode used for analysis of polar compounds that are difficult to retain in reversed phase column. Polar compounds elute later than hydrophobic compound. In case of analysis of hydrophilic compounds, ion pair reagents are often used in order to retain in reversed phase columns, but in HILIC it is possible to retain and separate without using this ion pair reagent.
Specifications
Base material | Functional group |
Particle size | Pore size | Surface area | pH range |
High purity totally spherical porous silica gel | β-cyclodextrin | 5µm | 120Å | 300m2/g | 2-7 |
High purity totally spherical porous silica gel | β-cyclodextrin | 2µm | 120Å | 300m2/g | 2-7 |
- Model: LCC-13311-151
- Manufactured by: Shinwa Chemical Industries Ltd