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Shodex HPLC Column OHpak LB-802.5 ID 8,0mm L 300mm - F6429206

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OHpak SB-800 / OHpak LB-800 columns separation by size for a variety of molecular weights

OHpak LB-802.5 HPLC Standard GFC column to be used with light scattering detector - 6μm - 8.0mm I.D. x 300mm Length

Shodex Product Number: F6429206

The polymer-based SEC (GFC) column for modified proteins, PEGs, polysaccharides, surfactants

Specifications: 

Description OHpak LB-802.5 8x300mm
Chemistry Producers Term LB-802.5
Gel material Polyhydroxymethacrylate
Separation Size Exclusion Chromatography (SEC) / Reversed Phase / Gel Filteration Chromatography (GFC)
Plates per column ≥ 16,000
Pore size 200 Å
Housing material steel
Maximum pressure 5.5 MPa (55 bar)
Usual flow rate 0.5 to 1.0 mL/min
Maximum flow rate 1.2 mL/min
Temperature range 4 to 80°C
pH range 3 to 10
Salt concentration ≤ 0.5 M
Shipping solvent H2O
MW range 500 - 10,000
Exclusion limit 10,000
MALS noise ≤ 5.0 × 10-5 V
Internal Diameter 8 mm
Length 300 mm
Brand OHPAK
Particle Size 6 µm
USP Class L25 / L89 / L37

OHpak LB-800 series [for MALS] Features

  • Polymer-based packed columns for aqueous SEC (GFC) analysis
  • Low column bleeding allows its use with light scattering detectors
  • The eluent can be replaced with DMF enabling the analysis of polar polymers
  • LB-802.5 (exclusion limit: about 10,000) 
  • Fulfills USP L25 requirements - Packing having the capacity to separate compounds with a molecular weight range from 100-5000 (as determined by polyethylene oxide), applied to neutral, anionic, and cationic water-soluble polymers. A polymethacrylate resin base, cross-linked with polyhydroxylated ether (surface contained some residual carboxyl functional groups) was found suitable.
  • Fulfills USP L89 requirements - Packing having the capacity to separate compounds with a molecular weight range from 100 to 3000 (as determined by polyethylene oxide), applied to neutral and anionic water-soluble polymers. A polymethacrylate resin base, cross-linked with polyhydroxylate ether (surface contains some residual cationic functional groups).

LB-800 series Possible Applications:

  • Aqueous SEC (GFC) - Application: Biological macromolecules (High MW range) - Eluent: Buffer etc.
  • Aqueous SEC (GFC) - Application: Water-soluble polymers (Polyacrylamide, etc.) - Eluent: Water, buffer and aqueous salt solution, etc.
  • Organic SEC (GPC) - Application:  Polar polymers (Polyimides etc.) -  Eluent: DMF

OHpak LB-802.5 Possible Applications:

  • Absolute Molecular Weight Determination of PMMA (LB-802.5)
  • Oligosaccharides - SEC
  • Hormones / Neurotransmitters - Reversed phase

GFC columns to be used with light scattering detector | Standard columns | Base Material: Polyhydroxymethacrylate | Usable pH Range: pH3 - 10

Product Code Product Name Plate Number
(TP/column)
Particle Size
(μm)
Pore Size
(Å)
Column Size (mm)
I.D. x Length
Shipping Solvent
F6429206 OHpak LB-802.5 ≥ 16,000 6 200 8.0 x 300 H2O
F6429201 OHpak LB-803 ≥ 16,000 6 800 8.0 x 300 H2O
F6429204 OHpak LB-804 ≥ 16,000 10 2,000 8.0 x 300 H2O
F6429203 OHpak LB-805 ≥ 12,000 13 7,000 8.0 x 300 H2O
F6429205 OHpak LB-806 ≥ 12,000 13 15,000 8.0 x 300 H2O
F6429202 OHpak LB-806M ≥ 12,000 13 15,000 8.0 x 300 H2O
F6709434 OHpak LB-G 6B (guard column) 13   6.0 x 50 H2O

LB-806M is a mixed-gel column capable of analyzing samples Base Material: Polyhydroxymethacrylate
over a wide range of molecular weight distribution.


  • Model: F6429206
  • Manufactured by: Showa Denko