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Shodex HPLC Column Asahipak ODP-50 10E ID 10,0mm L 250mm - F6820001

Price: £4,525.61


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Asahipak ODP-50 columns with C18 groups

Asahipak ODP-50 10E Preparative HPLC Column | 5μm - 10.0mm I.D. x 250 Length

Shodex Product Code: F6820001

Polymer-based Octadecyl C18 Reversed Phase Chromatography Columns

Specifications:

Description Asahipak ODP-50 10E 250x10mm
Quantity 1 pc
Chemistry Producers Term ODP
Plate Number (TP/column) ≥ 10,000
Matrix Polyvinyl alcohol
Functional Group Octadecyl
Mode of Separation Polymer based Preparative Column
Shipping Solvent H2O/CH3CN = 35/65
Housing material steel
Carbon load 17%
Temperature range 4 to 60°C
Salt concentration ≤ 0.1 M
pH range 2 to 13
Pore size 250 Å
Internal Diameter 10 mm
Length 250 mm
Brand Asahipak
Particle Morphology Polyvinyl alcohol
Particle Size 5 µm
USP L67

Asahipak ODP-50 columns

The Shodex Asahipak ODP-50 series are columns for reversed phase chromatography. These columns are packed with spherical porous polymer having octadecyl (C18) groups, and perform similar to general silica-based C18 ODS columns. These columns are compatible with not only water or polar organic solvents, but also the wide pH range between 2 to 13. There are ideal for LC-MS/MS analysis.

  • Relatively large pore size is suitable for the analysis of amino acids, peptides, and proteins
  • Usable in a wide pH range from pH 2 to 13
  • Usable in 100 % water and buffer solution
  • Best used for the analysis of basic substances
  • USP L67 - Porous vinyl alcohol copolymer with a C18 alkyl group attached to the hydroxyl group of the polymer, 2 to 10 μm in diameter.
  • Suitable for: Proteins Peptides - Reversed phase |  Amino acids - Reversed phase |  Drugs Metabolites - Reversed phase | Water-soluble vitamins - Reversed phase |  Fat-soluble vitamins - Reversed phase | Hormones / Neurotransmitters - Reversed phase | Lipids - Reversed phase | 

Over 60% of all HPLC applications are Reversed Phase (RP) separations with C18 functional groups. Spherical silica particles proved their use as base material in HPLC columns for many years. To minimize the negative effects of residual silanol groups on the surface, researchers tried end-capping or polymeric coating of the particle and developed hybrid materials.

Here we introduce an alternative for these ODS (octadecyl-bonded silica) RP columns:

A polymer particle (for example polyvinyl alcohol) with 5 μm particle size is modified with C18 alkyl chains as functional groups and used as stationary phase. This HPLC column has greater long-term pH stability from 2 to 13. It contains no silanol groups and provides higher resolution for basic substances, for example drugs with tertiary amines (no peak tailing due to unwanted interactions).

Also the polarity of the polymer gel is slightly more hydrophilic which gives more retention of polar compounds at the beginning of the chromatogram. The polymer-based RP material can be used in 100% water or buffer and a variety of organic solvents (isocratic and gradient separation). It has a pore size of 250 Å which makes it suitable for the analysis of proteins and peptides. The very low bleeding is advantageous for the hyphenation to mass spectrometry (MS) or particle-sensitive light scattering detectors. A C18 column with a stationary phase made of polymer offers more than double the life time compared to silica, because it is chemically more resistant.

Shodex is specialized in polymer-based packing material for Reversed Phase chromatography. The modern column Asahipak ODP-50 (octadecyl-bonded polymer) combines all the advantages of a polymeric RP-C18 column. It is used for small organic molecules, pharmaceuticals, pesticides, hormones, proteins, vitamins and basic drugs.

ODP-50 Range - Base Material: Polyvinyl alcohol

Product Code Product Name Plate Number (TP/column) Functional Group Particle Size (µm) Pore Size (Å) Column Size (mm) I.D. x Length Shipping Solvent
Standard columns
F7620002 Asahipak ODP-50 6D ≥ 9,000 Octadecyl C18 5 250 6.0 x 150 H2O/CH3CN = 35/65
F7620001 Asahipak ODP-50 6E ≥ 14,000 Octadecyl C18 5 250 6.0 x 250 H2O/CH3CN = 35/65
F6710001 Asahipak ODP-50G 6A (guard column) Octadecyl C18 5 6.0 x 10 H2O/CH3CN = 35/65
F6710023 Asahipak ODP-50 4B ≥ 2,500 Octadecyl C18 5 250 4.6 x 50 H2O/CH3CN = 35/65
F7620004 Asahipak ODP-50 4D ≥ 9,000 Octadecyl C18 5 250 4.6 x 150 H2O/CH3CN = 35/65
F7620003 Asahipak ODP-50 4E ≥ 14,000 Octadecyl C18 5 250 4.6 x 250 H2O/CH3CN = 35/65
F6710022 Asahipak ODP-50G 4A (guard column) Octadecyl C18 5 4.6 x 10 H2O/CH3CN = 35/65
F7620008 Asahipak C4P-50 4D ≥ 6,000 Butyl C4 5 250 4.6 x 150 H2O/CH3CN = 35/65
F6710003 Asahipak C4P-50G 4A (guard column) Butyl C4 5 4.6 x 10 H2O/CH3CN = 35/65
Semi-micro columns
F7620009 Asahipak ODP-50 2D ≥ 5,000 Octadecyl C18 5 250 2.0 x 150 H2O/CH3CN = 35/65
F6713001 Asahipak ODP-50G 2A (guard column) Octadecyl C18 5 - 2.0 x 10 H2O/CH3CN = 35/65
Preparative columns [ Preparative columns are made to order. ]
F6820001 Asahipak ODP-50 10E ≥ 10,000 Octadecyl C18 5 10.0 x 250 H2O/CH3CN = 35/65
F6820035 Asahipak ODP-90 20F ≥ 9,000 Octadecyl C18 9 20.0 x 300 H2O/CH3CN = 35/65
F6710004 Asahipak ODP-130G 7B (guard column) Octadecyl C18 13 7.5 x 50 H2O/CH3CN = 35/65

 


  • Model: F6820001
  • Manufactured by: Showa Denko