Ion Exchange Chromatography
TSKgel SP-STAT Cation Exchange Column, 7 μm, ID 4.6 mm x Length 10 cm
TSKgel SP-STAT columns are packed with 7 and 10 µm hydrophilic non-porous resin particles of which the surface consists of an open access network of multi-layered cation exchange groups. The innovative bonding chemistry, combined with a relatively large particle size, results in a respectable loading capacity and a low operating pressure.
Applications for the TSKgel SP-STAT columns include the separation of proteins, protein aggregates, charge isomers of monoclonal antibodies, PEGylated proteins, and peptide digests.
Product Attributes: Matrix: hydrophilic polymer Particle size (mean): 7 and 10 μm Pore size: non-porous Functional group: sulfopropyl Counter ion: Na pH range: 3.0-10.0 Static binding capacity (mg
Lysozyme/mg dry gel):
ca. 15 (7 µm)
ca. 10 (10 µm)
Small ion capacity: 23 ueq/g dry gel pKa: 4.0
Monitoring of protein PEGylation by ion-exchange chromatography on TSKgel SP-STAT
Conversion ratio of peak area
- Model: 21964
- Manufactured by: Tosoh BioScience