Gel Filtration Chromatography
TSKgel PWL Aqueous SEC Guard Column for G2000PW Columns, 13 μm, ID 7.5 mm x Length 7.5 cm (for P/Ns: 05761 and 05105)
Tosoh's polymer-based gel filtration columns are either prepared from polymethacrylate resin (TSKgel PW, PWXL and PWXL-CP) or from hydroxylated polyvinyl (TSKgel Alpha and SuperAW) resin. Each resin type can be synthesized in a wide variety of different pore sizes. The four major product groups also differ from one another in average particle size. A more important distinction is, however, the range of solvents that can be used with each product line.
Hydroxylated polymethacrylate beads
- From water to 20% (under some conditions 50%) organic solvents
- TSKgel PWXL and
TSKgel PWXL-CP, 7-13 μm
- TSKgel PW, 12-17 μm
Hydroxylated polyvinyl beads
- Water and most polar organic solvents
- TSKgel SuperAW, 4-9 μm
- TSKgel Alpha, 7-13 μm
Each product group contains columns with distinctly different pore sizes, and one mixed-bed column.
TSKgel PW columns are composed of spherical, hydrophilic polymethacrylate beads. Particle sizes range from 12 µm for the smaller pore size columns to 17 µm for the larger pore size columns. TSKgel G2000PW is ideally suited for analysis of water-soluble polymers with molecular weights up to 2,000 Da.
|Base material||hydroxylated polymethacrylate|
|Particle size (mean):||12 μm|
|Pore size:||125 Å|
|Calibration range: ||up to 2,000 Da (polyethylene |
glycols and oxides)
|Max. % Organic||20%|
Polyethylene glycol and oxide calibration curves for TSKgel PW columns