Waters ACQUITY Premier BEH Columns

BEH (Ethylene Bridged Hybrid) Technology

In 1999, Waters launched the XTerra family of HPLC columns featuring first generation hybrid particle technology (HPT). HPT enabled XTerra columns to become one of the most successful column products in the history of Waters. In HPT, the best properties of inorganic (silica) and organic (polymeric) packings are combined to produce a material that has superior mechanical strength, efficiency, high pH stability and peak shape for bases. The first generation methyl hybrid particles of XTerra columns did not possess the mechanical strength or efficiency necessary to fully realize the potential speed, sensitivity and resolution capabilities of UPLC technology. Therefore, a new pressure-tolerant particle needed to be created. A new, second generation hybrid material was developed which utilizes an ethylene bridged hybrid (BEH) structure. Compared to the first generation methyl hybrid particle of XTerra columns, the BEH particle of ACQUITY UPLC BEH columns exhibits improved efficiency, strength and pH range. BEH Technology™ is a key enabler behind the speed, sensitivity and resolution of UPLC separations.

Waters ACQUITY Premier BEH columns are chromatography columns that use BEH technology to separate polar compounds. They are available in different types, including amide, oligonucleotide, peptide, and shield RP18.

Features

• pH stability: BEH columns are stable over a wide pH range
• Temperature stability: BEH columns are stable over a range of temperatures
• Low non-specific adsorption: MaxPeak Premier surface technology reduces unwanted interactions between the analyte and the surface
• Reproducibility: BEH columns offer high reproducibility
• Peak shape: BEH columns offer good peak shape

Types of BEH columns

• ACQUITY Premier BEH Amide Column: Retains polar compounds like carbohydrates and sugars
• ACQUITY Premier Oligonucleotide BEH C18 Column: Separates oligonucleotides using ion-pairing reversed-phase (IP-RP) chromatography
• ACQUITY Premier Peptide BEH C18 Column: Separates peptides using peptide separation technology
• ACQUITY Premier BEH Shield RP18 Column: A type of BEH column

__________________________________________________________________________________________