Waters ACQUITY UPLC HSS T3 Peptide Column, 100A, 1.8 um, 2.1 mm X 150 mm, 1K - 15K, 3/pk - 186008782

Waters™ ACQUITY UPLC Peptide HSS T3 Column, 100Å, 1.8 µm, 2.1 mm X 150 mm, 1K - 15K, 3/pk

Product Number: 186008782

Specifications:

Peptide HSS T3 Columns are designed for separations where silica-based selectivities are desired or when increased retention of hydrophilic peptides are required.

The universal, silica-based bonded phase used for the ACQUITY HSS T3 sorbents is compatible with 100% aqueous mobile phase and should be your first choice when developing separations for polar and non-polar compounds. The ACQUITY Premier HSS T3 UPLC Columns exhibit ultra-low MS bleed and are fully scalable and transferable to HPLC separations. MaxPeak High Performance Surface hardware technology significantly reduces unwanted analyte/surface interactions that can lead to poor peak shape and losses in signal intensity. The VanGuard FIT Column design protects the column from contaminants and increases column lifetime.

High Strength Silica [HSS] Particle Technology

To complement Waters revolutionary Hybrid Particle Technology [HPT], a mechanically tolerant, silica-based material was designed to withstand UPLC pressures. High Strength Silica [HSS] particle technology was born from an innovative synthetic process that significantly increases the mechanical stability of silica while maintaining pore volumes similar to that of HPLC silica-based materials. The result is a novel particle technology that provides increased retentivity compared to hybrid particles while serving as the ideal substrate to create stationary phases that provide alternate selectivity.

ACQUITY UPLC columns

ACQUITY UPLC columns are the most technologically advanced LC columns ever created and are the only columns designed, tested and certified to withstand the pressures of UPLC Technology. Based upon the highly efficient 1.7 µm Bridged Ethyl Hybrid (BEH) particles and 1.8 µm High Strength Silica (HSS) particles, these columns are the first and only columns that allow separation scientists to achieve maximum speed, sensitivity and resolution without compromise. The particles and chemistries of the ACQUITY UPLC column family were carefully chosen to produce the ideal combination of ultra-performance efficiencies, wide pH range and complementary selectivities. ACQUITY UPLC BEH columns and ACQUITY UPLC HSS columns are the only columns recommended for use with ACQUITY UPLC Systems.

There is more to creating a UPLC particle than synthesizing a small particle. Many HPLC particles do not possess the mechanical stability and structural integrity to withstand UPLC operating pressures (e.g., 15000 psi/1000 bar). Why is pressure tolerance important? In order to realize the efficiency gains of sub-2 μm particles, the ability to routinely operate at higher linear velocities (e.g., higher flow rates) is required. These higher linear velocities combined with small, sub-2 μm particles result in higher operating backpressures. Waters has created two highly efficient, pressure-tolerant UPLC particles: the 1.7 μm Ethylene Bridged Hybrid (BEH) particle and the 1.8 μm High Strength Silica (HSS) particle.

The first ACQUITY UPLC particle created was the 1.7 μm Ethylene Bridged Hybrid (BEH) particle. This second generation hybrid particle is one of the key enablers behind UPLC technology and is available in five column chemistries: C18, C8, Shield RP18, Phenyl and HILIC. Because this is a hybrid particle, a wider usable pH range (up to pH 1-12) makes method development faster and easier. BEH particles are also available in HPLC particle sizes (2.5, 3.5, 5 and 10 μm) in the XBridge family of HPLC columns, thus allowing seamless transfer between HPLC and UPLC separations.