Waters ACQUITY UPLC Peptide HSS T3 Column, 100A, 1.8 um, 1 mm x 150 mm, 1/pk with Cytochrome C Digestion Standard - 176003994

Waters™ ACQUITY UPLC Peptide HSS T3 Column, 100 Å, 1.8 µm, 1 mm x 150 mm, 1/pk with Cytochrome C Digestion Standard

Product Number: 176003994

Specifications:

This kit includes one Peptide HSS T3 column and one vial of Cytochrome C Digestion Standard (P/N 186006371). The standard is included to be able to immediately benchmark the column upon receipt and can be reordered as a separate part for continued monitoring. Each batch of digestion standard is prepared under strict quality control criteria confirmed on the included certificate of analysis.

Cytochrome C Digestion Standard

The Cytochrome C Digestion Standard was prepared by digesting Bovine Heart Cytochrome C (Uniprot # P62894) with sequencing grade trypsin. Each batch of digestion standard is prepared under strict quality control criteria confirmed on the included certificate of analysis. Protein digestion standards may be used for a variety of applications, including evaluation and benchmarking of HPLC and UPLC columns or evaluation of mass spectrometry and chromatography instrumentation performance.

High Strength Silica [HSS] Particle Technology

To complement Waters revolutionary Hybrid Particle Technology [HPT], a mechanically tolerant, silica-based material was designed to withstand UPLC pressures. High Strength Silica [HSS] particle technology was born from an innovative synthetic process that significantly increases the mechanical stability of silica while maintaining pore volumes similar to that of HPLC silica-based materials. T he result is a novel particle technology that provides increased retentivity compared to hybrid particles while serving as the ideal substrate to create stationary phases that provide alternate selectivity.

As more separation scientists around the world realize the benefits of UPLC technology in their applications, Waters continues to provide additional UPLC particle and chemistry solutions to meet these demands. Waters material scientists developed a new High Strength Silica (HSS) particle with the high mechanical stability and appropriate morphology necessary to provide long column lifetimes and UPLC efficiencies at pressures up to 15000 psi (1000 bar). This 1.8 μm UPLC HSS particle is designed and tested specifically for use in UPLC separations.

ACQUITY UPLC columns

ACQUITY UPLC columns are the most technologically advanced LC columns ever created and are the only columns designed, tested and certified to withstand the pressures of UPLC Technology. Based upon the highly efficient 1.7 µm Bridged Ethyl Hybrid (BEH) particles and 1.8 µm High Strength Silica (HSS) particles, these columns are the first and only columns that allow separation scientists to achieve maximum speed, sensitivity and resolution without compromise. The particles and chemistries of the ACQUITY UPLC column family were carefully chosen to produce the ideal combination of ultra-performance efficiencies, wide pH range and complementary selectivities. ACQUITY UPLC BEH columns and ACQUITY UPLC HSS columns are the only columns recommended for use with ACQUITY UPLC Systems.

There is more to creating a UPLC particle than synthesizing a small particle. Many HPLC particles do not possess the mechanical stability and structural integrity to withstand UPLC operating pressures (e.g., 15000 psi/1000 bar). Why is pressure tolerance important? In order to realize the efficiency gains of sub-2 μm particles, the ability to routinely operate at higher linear velocities (e.g., higher flow rates) is required. These higher linear velocities combined with small, sub-2 μm particles result in higher operating backpressures. Waters has created two highly efficient, pressure-tolerant UPLC particles: the 1.7 μm Ethylene Bridged Hybrid (BEH) particle and the 1.8 μm High Strength Silica (HSS) particle.

The first ACQUITY UPLC particle created was the 1.7 μm Ethylene Bridged Hybrid (BEH) particle. This second generation hybrid particle is one of the key enablers behind UPLC technology and is available in five column chemistries: C18, C8, Shield RP18, Phenyl and HILIC. Because this is a hybrid particle, a wider usable pH range (up to pH 1-12) makes method development faster and easier. BEH particles are also available in HPLC particle sizes (2.5, 3.5, 5 and 10 μm) in the XBridge family of HPLC columns, thus allowing seamless transfer between HPLC and UPLC separations.

ACQUITY UPLC HSS column chemistries include HSS C18, HSS C18 SB (Selectivity for Bases) and T3. The HSS C18 chemistry is a fully endcapped, ultra-performance, general purpose C18 bonded phase that provides superior peak shape for bases, increased retentivity (vs. ACQUITY UPLC BEH C18 columns), and extremely long lifetimes under acidic conditions. The HSS C18 SB (Selectivity for Bases) chemistry is an unendcapped C18 bonded phase designed and optimized for low pH method development and offers alternate selectivities, especially for basic com- pounds, as compared to most modern, high coverage C18 chemistries. The HSS T3 chemistry is an aqueous mobile phase compatible C18 bonded phase that is designed to retain and separate small, water soluble, polar organic molecules, much like Atlantis T3 HPLC columns.

Related Products

• 186002329 - MassPREP BSA Digestion Standard
• 186008757 - ACQUITY UPLC Peptide HSS T3 VanGuard Pre-column, 100Å, 1.8 µm, 2.1 mm X 5 mm, 1K - 15K, 3/pk
• 186002326 - MassPREP Phosphorylase b Standard
• 186002328 - MassPREP ADH Digestion Standard
• 186002327 - MassPREP Bovine Hemoglobin Standard
• 186006371 - Cytochrome C Digestion Standard