Waters ACQUITY UPLC Peptide HSS T3 Column, 100A, 1.8 um, 2.1 mm X 100 mm, 1K - 15K, 1/pk - 186008755

Waters™ ACQUITY UPLC Peptide HSS T3 Column, 100Å, 1.8 µm, 2.1 mm X 100 mm, 1K - 15K, 1/pk

Product Number: 186008755

Specifications:

Peptide HSS T3 Columns are designed for separations where silica-based selectivities are desired or when increased retention of hydrophilic peptides are required.

This particular ACQUITY UPLC Peptide HSS T3 Column offers a minimum of 2, and a maximum of 8 pH.

The ACQUITY UPLC Peptide HSS T3 Column is able to sustain up to 45 C.

ACQUITY UPLC Peptide HSS T3 Column, 100Å, 1.8 µm, 2.1 mm X 100 mm, 1K - 15K, 1/pk

To satisfy a wide range of chromatographic applications, rely on the great value provided by the ACQUITY UPLC Peptide HSS T3 Column. The lab equipment makes it simple to switch methods while enhancing productivity and maintaining or increasing the resolution across components. Recognizing that the range of analyte polarity can make developing chromatographic methods problematic, Waters created the ACQUITY UPLC Peptide HSS T3 Column to allow a single chromatographic method to be used to examine a variety of formulation compositions. Because of the ACQUITY UPLC Peptide HSS T3 Column's remarkable characteristics, you will not only be able to meet the challenges of the laboratory, but you will also be more productive and successful in your work.

To handle the challenges of liquid chromatography with ease and confidence, add the ACQUITY UPLC Peptide HSS T3 Column to your lab equipment collection. The analytical column has a low ligand density and is ideally suited for reversed-phase liquid chromatography for better retention of polar chemicals and metabolites. The analytes will be able to reach the material's pore structure by using the ACQUITY UPLC Peptide HSS T3 Column. This eliminates the necessity for ion-pair reagents in the retention of polar and hydrophobic compounds.

The Waters HSS (High Strength Silica) particle technology used in the ACQUITY UPLC Peptide HSS T3 Column was developed specifically to withstand UPLC pressures by combining a mechanically tolerant silica-based material with an innovative synthetic process that significantly increases silica's mechanical stability. When compared to hybrid particle technology, this results in higher retentivity and alternative selectivity.

Because the ACQUITY UPLC Peptide HSS T3 Columns use Peptide Separation Technology (PST), which is based on BEH Technology particles, they meet the stringent BEH Technology particle criteria and are QC verified with a peptide map. As a result, well-developed peptide separations are stable, and many distinct samples utilized in proteomics, protein characterization, and peptide synthesis behave predictably. Over a wide range of peptide characteristics and application requirements, the surface chemistry produces crisp symmetrical peaks.

High Strength Silica [HSS] Particle Technology

To complement Waters revolutionary Hybrid Particle Technology [HPT], a mechanically tolerant, silica-based material was designed to withstand UPLC pressures. High Strength Silica [HSS] particle technology was born from an innovative synthetic process that significantly increases the mechanical stability of silica while maintaining pore volumes similar to that of HPLC silica-based materials. T he result is a novel particle technology that provides increased retentivity compared to hybrid particles while serving as the ideal substrate to create stationary phases that provide alternate selectivity.

As more separation scientists around the world realize the benefits of UPLC technology in their applications, Waters continues to provide additional UPLC particle and chemistry solutions to meet these demands. Waters material scientists developed a new High Strength Silica (HSS) particle with the high mechanical stability and appropriate morphology necessary to provide long column lifetimes and UPLC efficiencies at pressures up to 15000 psi (1000 bar). This 1.8 μm UPLC HSS particle is designed and tested specifically for use in UPLC separations.

ACQUITY UPLC columns

ACQUITY UPLC columns are the most technologically advanced LC columns ever created and are the only columns designed, tested and certified to withstand the pressures of UPLC Technology. Based upon the highly efficient 1.7 µm Bridged Ethyl Hybrid (BEH) particles and 1.8 µm High Strength Silica (HSS) particles, these columns are the first and only columns that allow separation scientists to achieve maximum speed, sensitivity and resolution without compromise. The particles and chemistries of the ACQUITY UPLC column family were carefully chosen to produce the ideal combination of ultra-performance efficiencies, wide pH range and complementary selectivities. ACQUITY UPLC BEH columns and ACQUITY UPLC HSS columns are the only columns recommended for use with ACQUITY UPLC Systems.

There is more to creating a UPLC particle than synthesizing a small particle. Many HPLC particles do not possess the mechanical stability and structural integrity to withstand UPLC operating pressures (e.g., 15000 psi/1000 bar). Why is pressure tolerance important? In order to realize the efficiency gains of sub-2 μm particles, the ability to routinely operate at higher linear velocities (e.g., higher flow rates) is required. These higher linear velocities combined with small, sub-2 μm particles result in higher operating backpressures. Waters has created two highly efficient, pressure-tolerant UPLC particles: the 1.7 μm Ethylene Bridged Hybrid (BEH) particle and the 1.8 μm High Strength Silica (HSS) particle.

The first ACQUITY UPLC particle created was the 1.7 μm Ethylene Bridged Hybrid (BEH) particle. This second generation hybrid particle is one of the key enablers behind UPLC technology and is available in five column chemistries: C18, C8, Shield RP18, Phenyl and HILIC. Because this is a hybrid particle, a wider usable pH range (up to pH 1-12) makes method development faster and easier. BEH particles are also available in HPLC particle sizes (2.5, 3.5, 5 and 10 μm) in the XBridge family of HPLC columns, thus allowing seamless transfer between HPLC and UPLC separations.

ACQUITY UPLC HSS column chemistries include HSS C18, HSS C18 SB (Selectivity for Bases) and T3. The HSS C18 chemistry is a fully endcapped, ultra-performance, general purpose C18 bonded phase that provides superior peak shape for bases, increased retentivity (vs. ACQUITY UPLC BEH C18 columns), and extremely long lifetimes under acidic conditions. The HSS C18 SB (Selectivity for Bases) chemistry is an unendcapped C18 bonded phase designed and optimized for low pH method development and offers alternate selectivities, especially for basic com- pounds, as compared to most modern, high coverage C18 chemistries. The HSS T3 chemistry is an aqueous mobile phase compatible C18 bonded phase that is designed to retain and separate small, water soluble, polar organic molecules, much like Atlantis T3 HPLC columns.

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