Waters XBridge Premier BEH Amide Column, 3.5 µm, VanGuard FIT Method Validation Kit 2.1 x 50 mm, 3/pk
Part Number: 186011010
The method validation kit (MVK) contains three columns each packed with a different lot of material. XBridge Premier BEH Amide Columns retain extremely polar compounds, including carbohydrates and sugars. Based on BEH technology, the XBridge Amide Columns offer a chemically stable, trifunctionally bonded amide phase that is stable from pH 2 to 11 to enable the separation of polar analytes that span a wide range of polarity, structural moiety and pKa. XBridge Premier 3.5 µm Columns enable exceptional separation performance, robustness and throughput for HPLC assays while enabling a seamless transition path toward UPLC adoption. XBridge Premier Columns use MaxPeak High Performance Surface hardware technology which significantly reduces unwanted analyte/surface interactions that can lead to poor peak shape and losses in signal intensity.
BEH (Ethylene Bridged Hybrid) Technology
Ethylene Bridged Hybrid (BEH) particle technology ensures maximum column performance and longer column lifetimes under all chromatographic conditions. The hybrid particle technology offers many advantages over conventional silica-based particles, including the ability to control silanol activity for better reproducibility, peak shape, and efficiency. The BEH particle is created from two high purity monomers: tetraethoxysilane (TEOS) and bis(triethoxysilyl) ethane (BTEE), which results in highly stable, pH resistant, and mechanically strong particles that set the performance standard for method development. BEH particle technology allows for seamless transfer from analytical to preparative separations.
The advantages of BEH columns include:
- Rugged hybrid organic/inorganic base particle
- Improved peak shape for bases-low silanol activity
- Wider operating pH range for method development flexibly
- Mechanical strength for maximum column efficiency at UHPLC operating pressures
- Available Particle Sizes: 1.7, 2.5, 3.5, 5, and 10 µm
- Column Chemistries: C18, C18 AX, Shield RP18, C8, Phenyl, Amide, Z-HILIC, HILIC
Related Products:
- 186010962 - XBridge Premier BEH Amide VanGuard FIT Column, 3.5 µm, 2.1 x 50 mm, 1/pk
- 186006360 - Neutrals QC Reference Material
- 186006363 - Reversed-Phase QC Reference Material
Specifications:
Chemistry | Amide |
Separation Mode | Hydrophilic Interaction (HILIC) |
Particle Substrate | Hybrid |
pH Range Min | 2 pH |
pH Range Max | 11 pH |
Temperature Limits | 90 C |
Maximum Pressure | 6000 psi (415 Bar) |
Endcapped | No |
Silanol Activity | Low |
Particle Shape | Spherical |
Particle Size | 3.5 µm |
Endfitting Type | Parker-style |
Pore Size | 130 Å |
Format | Method Validation Kit |
Surface Area | 185 |
System | HPLC |
Particle Technology | BEH |
Technique | LC |
USP Classification | L68 |
Inner Diameter | 2.1 mm |
Length | 50 mm |
Carbon Load | 12% |
UNSPSC | 41115709 |
Application | Small Molecule |
Brand | XBridge |
Product Type | Column Kits |
Units per Package | 3 pk |
- Model: 186011010
- Manufactured by: Waters