Waters XBridge Premier BEH Amide VanGuard FIT Column, 3.5 µm, 4.6 x 250 mm, 1/pk
Part Number: 186010973
XBridge Premier BEH Amide Columns retain extremely polar compounds, including carbohydrates and sugars. Based on BEH technology, the XBridge Amide Columns offer a chemically stable, trifunctionally bonded amide phase that is stable from pH 2 to 11 to enable the separation of polar analytes that span a wide range of polarity, structural moiety and pKa. XBridge Premier 3.5 µm Columns enable exceptional separation performance, robustness and throughput for HPLC assays while enabling a seamless transition path toward UPLC adoption. XBridge Premier Columns use MaxPeak High Performance Surface hardware technology which significantly reduces unwanted analyte/surface interactions that can lead to poor peak shape and losses in signal intensity. The VanGuard FIT Column design protects the column from contaminants and increases column lifetime.
VanGuard Column Protection
Protect and prolong analytical column lifetime
Even under the best conditions, analytical LC columns deteriorate over time from chemical or particulate contamination. With VanGuard Column Protection products, you can now achieve maximum lifetime and performance from your columns.
VanGuard column products are optimized to protect and prolong analytical column lifetimes without compromising chromatographic performance. Available in a wide selection of particle sizes and stationary phases across HPLC, UHPLC, and UPLC, VanGuard Column Protection products are ideally suited for the physical and chemical protection of all Waters analytical columns.
BEH (Ethylene Bridged Hybrid) Technology
Ethylene Bridged Hybrid (BEH) particle technology ensures maximum column performance and longer column lifetimes under all chromatographic conditions. The hybrid particle technology offers many advantages over conventional silica-based particles, including the ability to control silanol activity for better reproducibility, peak shape, and efficiency. The BEH particle is created from two high purity monomers: tetraethoxysilane (TEOS) and bis(triethoxysilyl) ethane (BTEE), which results in highly stable, pH resistant, and mechanically strong particles that set the performance standard for method development. BEH particle technology allows for seamless transfer from analytical to preparative separations.
The advantages of BEH columns include:
- Rugged hybrid organic/inorganic base particle
- Improved peak shape for bases-low silanol activity
- Wider operating pH range for method development flexibly
- Mechanical strength for maximum column efficiency at UHPLC operating pressures
- Available Particle Sizes: 1.7, 2.5, 3.5, 5, and 10 µm
- Column Chemistries: C18, C18 AX, Shield RP18, C8, Phenyl, Amide, Z-HILIC, HILIC
Related Products:
- 186010974 - XBridge Premier BEH Amide VanGuard FIT Cartridge, 3.5 µm, 3.9 x 5 mm, 3/pk
- 186006360 - Neutrals QC Reference Material
- 186006363 - Reversed-Phase QC Reference Material
- 186010969 - XBridge Premier BEH Amide Column, 3.5 µm, 4.6 x 250mm, 1/pk
Specifications:
Chemistry | Amide |
Separation Mode | Hydrophilic Interaction (HILIC) |
Particle Substrate | Hybrid |
pH Range Min | 2 pH |
pH Range Max | 11 pH |
Temperature Limits | 90 C |
Maximum Pressure | 6000 psi (415 Bar) |
Endcapped | No |
Silanol Activity | Low |
Particle Shape | Spherical |
Particle Size | 3.5 µm |
Endfitting Type | Parker-style |
Pore Size | 130 Å |
Format | Premier Column |
Surface Area | 185 |
System | HPLC |
Particle Technology | BEH |
Technique | LC |
USP Classification | L68 |
Inner Diameter | 4.6 mm |
Length | 250 mm |
Carbon Load | 12% |
UNSPSC | 41115709 |
Application | Small Molecule |
Brand | XBridge |
Product Type | Columns |
Units per Package | 3 pk |
- Model: 186010973
- Manufactured by: Waters