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Waters XBridge Premier GTx BEH SEC Column, 450 Å, 2.5 µm, 4.6 x 300 mm, 1/pk with eConnect Column Tag - 186010585RF

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Waters XBridge Premier GTx BEH SEC Column, 450 Å, 2.5 µm, 4.6 x 300 mm, 1/pk with eConnect Column Tag

Product Number: 186010585RF

To help resolve uncertainty at the pace of gene therapy development, XBridge Premier GTx BEH SEC 450 Å, 2.5 µm, 4.6 x 300 mm column provides high resolution and fast size variant separations for AAVs and nucleic acids. AAV monomers and aggregates are ideally resolved using a 450 Å pore size. Unwanted secondary interactions that make it difficult to fully recover your sample and create robust, serotype independent, and stable platform methods are alleviated by hydrophilic MaxPeak High Performance Surface hardware. Industry-leading resolution without undesirable analyte shearing is provided by efficiently packed 2.5 µm particles. Suggested Use: Gel Filtration, Size Exclusion Chromatography, Aggregate, Monomer and Fragment analysis of AAVs and nucleic acids. This column comes equipped with an electronic eConnect column tag containing unique column-specific information. These NFC-enabled device-tagged columns were developed to automatically identify, track and digitally trace column use history. eConnect columns can be used on any HPLC, but its advantages are unlocked when used with the Waters Alliance iS and Alliance iS Bio System running Empower software.

Built with BEH for mechanical strength and chemical stability

Ethylene Bridged Hybrid (BEH) particle technology ensures maximum column performance and longer column lifetimes under all chromatographic conditions. The hybrid particle technology offers many advantages over conventional silica-based particles, including the ability to control silanol activity for better reproducibility, peak shape, and efficiency. The BEH particle is created from two high purity monomers: tetraethoxysilane (TEOS) and bis(triethoxysilyl) ethane (BTEE), which results in highly stable, pH resistant, and mechanically strong particles that set the performance standard for method development. BEH particle technology allows for seamless transfer from analytical to preparative separations.

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Specifications:

ChemistrySEC
Separation ModeSEC
Particle SubstrateHybrid
pH Range Min2.5 pH
pH Range Max8 pH
Temperature Limits60 C
Maximum Pressure4600 psi (317 Bar)
EndcappedNo
Bonding TechnologyDiol (OH)
Silanol ActivityLow
Molecular Weight Range Min10000
Molecular Weight Range Max100000
Particle ShapeSpherical
Particle Size2.5 µm
Endfitting TypeParker-style
Pore Size450 Å
FormatPremier Column
Surface Area80
SystemHPLC, UHPLC, UPLC
Particle TechnologyBEH
USP ClassificationL33
Inner Diameter4.6 mm
Length300 mm
UNSPSC41115709
ApplicationGene Therapeutics
BrandXBridge
Product TypeColumns
Units per Package1 pk
eConnectYes

 


  • Model: 186010585RF
  • Manufactured by: Waters