Waters XBridge Premier GTx BEH SEC Column, 450 Å, 2.5 µm, 4.6 x 300 mm, 1/pk with eConnect Column Tag
Product Number: 186010585RF
To help resolve uncertainty at the pace of gene therapy development, XBridge Premier GTx BEH SEC 450 Å, 2.5 µm, 4.6 x 300 mm column provides high resolution and fast size variant separations for AAVs and nucleic acids. AAV monomers and aggregates are ideally resolved using a 450 Å pore size. Unwanted secondary interactions that make it difficult to fully recover your sample and create robust, serotype independent, and stable platform methods are alleviated by hydrophilic MaxPeak High Performance Surface hardware. Industry-leading resolution without undesirable analyte shearing is provided by efficiently packed 2.5 µm particles. Suggested Use: Gel Filtration, Size Exclusion Chromatography, Aggregate, Monomer and Fragment analysis of AAVs and nucleic acids. This column comes equipped with an electronic eConnect column tag containing unique column-specific information. These NFC-enabled device-tagged columns were developed to automatically identify, track and digitally trace column use history. eConnect columns can be used on any HPLC, but its advantages are unlocked when used with the Waters Alliance iS and Alliance iS Bio System running Empower software.
Built with BEH for mechanical strength and chemical stability
Ethylene Bridged Hybrid (BEH) particle technology ensures maximum column performance and longer column lifetimes under all chromatographic conditions. The hybrid particle technology offers many advantages over conventional silica-based particles, including the ability to control silanol activity for better reproducibility, peak shape, and efficiency. The BEH particle is created from two high purity monomers: tetraethoxysilane (TEOS) and bis(triethoxysilyl) ethane (BTEE), which results in highly stable, pH resistant, and mechanically strong particles that set the performance standard for method development. BEH particle technology allows for seamless transfer from analytical to preparative separations.
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Specifications:
Chemistry | SEC |
Separation Mode | SEC |
Particle Substrate | Hybrid |
pH Range Min | 2.5 pH |
pH Range Max | 8 pH |
Temperature Limits | 60 C |
Maximum Pressure | 4600 psi (317 Bar) |
Endcapped | No |
Bonding Technology | Diol (OH) |
Silanol Activity | Low |
Molecular Weight Range Min | 10000 |
Molecular Weight Range Max | 100000 |
Particle Shape | Spherical |
Particle Size | 2.5 µm |
Endfitting Type | Parker-style |
Pore Size | 450 Å |
Format | Premier Column |
Surface Area | 80 |
System | HPLC, UHPLC, UPLC |
Particle Technology | BEH |
USP Classification | L33 |
Inner Diameter | 4.6 mm |
Length | 300 mm |
UNSPSC | 41115709 |
Application | Gene Therapeutics |
Brand | XBridge |
Product Type | Columns |
Units per Package | 1 pk |
eConnect | Yes |
- Model: 186010585RF
- Manufactured by: Waters