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Waters XBridge Premier Peptide BEH C18 Column, 130 Å, 2.5 µm, 4.6 mm x 150 mm, 1/pk with eConnect Column Tag - 186009900RF

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Waters XBridge Premier Peptide BEH C18 Column, 130 Å, 2.5 µm, 4.6 mm x 150 mm, 1/pk with eConnect Column Tag

Product Number: 186009900RF

The XBridge Premier Peptide BEH C18 Column, 130 Å, 2.5 µm, 4.6 mm x 150 mm Columns have been specially optimized and QC tested with cytochrome c digest standards (p/n: 186006371) for peptide separations. The small-pore (130 Å) trifunctionally bonded BEH particle offers the widest usable pH range, superior low pH stability, and ultra-low column bleed to assay samples for proteomics, protein characterization, and peptide synthesis. This column is part of the MaxPeak Premier Class of Columns, which deliver the chromatographic performance expected from our particle technologies while increasing reproducibility, peak shape, and recovery for metal-sensitive analytes. Suggested Use: Peptide Separations, Peptide Analysis. This column comes equipped with an electronic eConnect column tag containing unique column-specific information. These NFC-enabled device-tagged columns were developed to automatically identify, track and digitally trace column use history. eConnect columns can be used on any HPLC, but its advantages are unlocked when used with the Waters Alliance iS and Alliance iS Bio System running Empower software

BEH (Ethylene Bridged Hybrid) Technology

In 1999, Waters launched the XTerra family of HPLC columns featuring first generation hybrid particle technology (HPT). HPT enabled XTerra columns to become one of the most successful column products in the history of Waters. In HPT, the best properties of inorganic (silica) and organic (polymeric) packings are combined to produce a material that has superior mechanical strength, efficiency, high pH stability and peak shape for bases. The first generation methyl hybrid particles of XTerra columns did not possess the mechanical strength or efficiency necessary to fully realize the potential speed, sensitivity and resolution capabilities of UPLC technology. Therefore, a new pressure-tolerant particle needed to be created. A new, second generation hybrid material was developed which utilizes an ethylene bridged hybrid (BEH) structure. Compared to the first generation methyl hybrid particle of XTerra columns, the BEH particle of ACQUITY UPLC BEH columns exhibits improved efficiency, strength and pH range. BEH Technology™ is a key enabler behind the speed, sensitivity and resolution of UPLC separations.

Specifications:

ChemistryC18
Separation ModeReversed Phase
Particle SubstrateHybrid
pH Range Min1 pH
pH Range Max12 pH
Maximum Pressure10000 psi (690 Bar)
EndcappedYes
Silanol ActivityLow
Particle ShapeSpherical
Particle Size2.5 µm
Endfitting TypeParker-style
Pore Size130 Å
FormatPremier Column
Surface Area185
SystemUHPLC, UPLC
Particle TechnologyBEH
TechniqueLC
USP ClassificationL1
Inner Diameter4.6 mm
Length150 mm
UNSPSC41115711
ApplicationPeptide
BrandXBridge
Product TypeColumns
eConnectYes


 


  • Model: 186009900RF
  • Manufactured by: Waters