Waters XBridge Premier Protein SEC Column with eConnect Column Tag, 250 Å, 2.5 µm, 4.6 x 300 mm, 1/pk - 186009960RF

Waters XBridge Premier Protein SEC Column, 250Å, 2.5 µm, 4.6 x 300 mm, 1/pk with eConnect Column Tag

Product Number: 186009960RF

XBridge Premier Protein SEC Column, 250 Å, 2.5 µm, 4.6 x 150 mm Columns help scientists obtain reliable protein size variant separations (10,000 to approx 650,000 Daltons) via the synergistic coupling of Waters MaxPeak Premier High Performance Surfaces and novel BEH-PEO Particle technologies. These advancements in SEC column hardware and SEC particle technology work to minimize non-desired secondary ionic or hydrophobic interactions between proteins and the column to minimize costly method development work by allowing chromatographers to use a "generic" or "platform-type" method for many samples. Each batch is specifically QC tested using Waters mAb Size Variant Standard (p/n: 186009429) to help ensure consistent performance for challenging size-based protein separations.

Note: The same column is also available with the Waters mAb Size Variant Standard at no additional cost. (p/n: 176005067).

Suggested Use: Gel Filtration, Size Exclusion Chromatography, Aggregate, Monomer and Fragment analysis, Antibody Conjugates. This column comes equipped with an electronic eConnect column tag containing unique column-specific information. These NFC-enabled device-tagged columns were developed to automatically identify, track and digitally trace column use history. eConnect columns can be used on any HPLC, but its advantages are unlocked when used with the Waters Alliance iS and Alliance iS Bio System running Empower software.

Protein Columns

Protein Analyses and Lab Scale Purifications

The development and successful commercialization of protein-based biopharmaceuticals and diagnostic reagents frequently depends on the ability to adequately characterize these complex biomolecules. Waters columns and methods can help solve your protein separation and characterization challenges.

Waters technology utilizes reversed-phase, ion-exchange, hydrophilic-interaction, and size-exclusion chromatography modes for peptide separation applications such as the analysis and purification of synthetic peptides, the characterization of complex proteolytic digests, and bioanalysis. These orthogonal separation techniques help provide the critical characterization data and isolated material required to produce the next generation drug.

SEC Columns

Size-based separations of peptides, proteins, nucleic acids, LNPs, and viral vectors
Get unmatched batch-to-batch reproducibility with the wide range of Waters Size-Exclusion Chromatography (SEC) Columns and standards for protein and peptide characterization.

SEC is a powerful technique for aggregate and fragment analysis in the research, development, and manufacturing of biotherapeutic proteins, such as insulin and monoclonal antibodies (mAb). With SEC, also known as gel filtration, you can separate proteins based on their size (hydrodynamic radii versus absolute molecular weight) in solution, with larger sized species eluting before smaller proteins.

The pore size and volume of the SEC particles used in the packed column drives this isocratic based technique, so the column you choose matters. As the molecular weight of the proteins of interest increases, SEC columns with comparatively larger pore size particles are selected.

Specifications