Waters High Sensitivity Filter Mixer, 425 µL - 205000403

Waters High Sensitivity Filter Mixer, 425µL

Part Number: 205000403

ACQUITY UPLC Mixer Options

The standard Waters ACQUITY UPLC System is equipped with 50 µL in-line mobile phase mixer. For demanding biopolymer separations (e.g., peptide mapping), use of a shallow gradients (e.g., 0.15% mobile phase B change per minute) is required. In these situations, it is recommended that the organic solvent concentration in mobile phase B be reduced by “premixing” with a measured amount of mobile phase A (e.g., mobile phase A= 0.1 M TEAA and mobile phase B= acetonitrile/0.1 M TEAA, 20/80, v/v).

Use of a 425 µL mixer (p/n: 205000403) specifically designed for shallow UPLC gradient separations is recommended when the solvent premixing technique (detailed above) is not used and when mobile phase B contains either 100% acetonitrile (for TEAA ion-pairing method) or 100% methanol (for TEA-HFIP ion-pairing method).