Biomiga BT549(Human breast cancer cell line) whole cell lysate - CL-003

Description :

Introduction

Biomiga provides researchers with a variety of ready-to-use cell line lysates for proteomics research. Biomiga lysates are available for applications including electrophoresis, western blot, immunoprecipitation, enzymatic activity analysis, protein-protein interaction analysis, and tissue specific expression identification. Please check product documentation to see recommended and tested applications for each cell line lysate.

Product Name 

BT549 (Human breast cancer cell line) Whole Cell Lysate

Product type 

Lysates

Description 

BT549 (Human breast cancer cell line) Whole Cell Lysate

Tested applications 

WB

Suggested applications 

WB, ELISA, Immunoprecipitation

Recommended dilutions
BT549 cell lysate is ready to run SDS-PAGE for Western blotting, 20 µg per lane is recommended for mini gel.

Relevance 

BT549 cell lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylene diamine tetra acetic acid, 1 mM phenyl methyl sulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecyl sulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay.

Purity 

Whole Cell Lysate

Form 

It is supplied as whole cell lysate, in RIPA buffer with protease inhibitors and phosphotase inhibitors.

Concentration 

2.000 mg/ml

Storage instructions 

Shipped on dry ice. Upon delivery aliquot and store at  -80°C. Avoid repeated freeze / thaw cycles.

Notes 

Cell line: BT549 (Human breast adenocarcinoma)
Growth media: RPMI1640 & 10% FCS (Fetal Calf serum)