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Biomiga Blood DNA Maxi Kit - GD2314-00

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Description :

Introduction 

The EZgeneTM Blood maxi gDNA kit provides a fast and easy method for isolating gDNA from blood. The system utilizes the reversible nucleic acid-binding properties of Biomiga's ezBind membrane and the speed of spin column technology to yield high quality gDNA with the OD260/280 ratio of 1.8-2.0. Up to 20 mL of fresh, frozen or anticoagulated whole blood treated with any common anticoagulant such as heparin, EDTA, or acid-citrate-dextrose can be readily processed at one time. This DNA Kit can also be used for the preparation of genomic DNA from buffy coat, serum, plasma, saliva, buccal swab and other body fluids. Purified DNA is ready for applications such as PCR, Southern Blotting, and Restriction Digestion.

The binding capacity per column is 1000 μg of gDNA. Use less than20 mL of whole Blood or buffy coat is recommended.

Storage and Stability

All EZgeneTM Blood maxi gDNA Kit components are guaranteed for at least 12 months from the date of purchase when stored as follows:

  • Reconstituted Protease K store at -20°C.
  • All other materials at room temperature (22-25°C).

Kit Contents

Catalog #

GD2314-00

GD2314-01

GD2314-02

Preps

2

10

25

ezBind DNA Maxi Columns

2

10

25

50 mL Collection Tubes

4

20

50

Buffer TL

10 mL

40 mL

100 mL

Buffer BL

15 mL

75 mL

175 mL

Buffer KB

22 mL

110 mL

260 mL

10 x Red Blood Cell Lysis Buffer

30 mL

160  mL

400 mL

DNA Wash Buffer

12 mL

50 mL

2 x 50 mL

Elution Buffer

20 mL

100 mL

250 mL

Protease K

12 mg

2x30 mg

5x30 mg

RNase A (20 mg/mL)

45 μL

210 μL

520 μL

Instruction Booklet

1

1

1

 

Before Starting

The kit is designed to be simple, fast, and reliable provided that all steps are followed diligently. Please read the entire booklet and get all necessary supplies and equipments.

Important

  • Reconstitute Protease K as follows: Add 600 μL (GD2314-00), 2x1.5 mL (GD2314-01), or 5x1.5 mL (GD2314-02) Elution Buffer and vortex the vial briefly before use. Aliquot and store vials of reconstituted Protease K at -20°C.
  • Dilute DNA Wash Buffer with absolute ethanol as follows:  Add 48 mL (GD2314-00) or 200 mL (GD2314-01) or 2x200 mL (GD2314-02) of absolute ethanol to each bottle. The final concentration is 70%.
  • Under cool ambient conditions, precipitates may form in Buffer BL. In case of such an event, heat the bottle at 37°C to dissolve before use.
  • Preheat the Elution Buffer at 70°C.

Materials provided by user

  • Centrifuge capable of 5,000 x g
  • Sterile 50 mL centrifuge tubes
  • Water bath set to 55°C and 70°C
  • Absolute ethanol

Safety Information

Buffer BL contains chaotropic salts, which may form reactive compounds when combines with bleach, Do not add bleach or acidic solutions directly to the preparation waste, ware gloves and protective eyewear when handling.


  • Model: GD2314-00
  • Manufactured by: Biomiga

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