Description : Introduction The EZgeneTM Blood maxi gDNA kit provides a fast and easy method for isolating gDNA from blood. The system utilizes the reversible nucleic acid-binding properties of Biomiga's ezBind membrane and the speed of spin column technology to yield high quality gDNA with the OD260/280 ratio of 1.8-2.0. Up to 20 mL of fresh, frozen or anticoagulated whole blood treated with any common anticoagulant such as heparin, EDTA, or acid-citrate-dextrose can be readily processed at one time. This DNA Kit can also be used for the preparation of genomic DNA from buffy coat, serum, plasma, saliva, buccal swab and other body fluids. Purified DNA is ready for applications such as PCR, Southern Blotting, and Restriction Digestion. The binding capacity per column is 1000 μg of gDNA. Use less than20 mL of whole Blood or buffy coat is recommended. Storage and Stability All EZgeneTM Blood maxi gDNA Kit components are guaranteed for at least 12 months from the date of purchase when stored as follows: Kit Contents Catalog # GD2314-00 GD2314-01 GD2314-02 Preps 2 10 25 ezBind DNA Maxi Columns 2 10 25 50 mL Collection Tubes 4 20 50 Buffer TL 10 mL 40 mL 100 mL Buffer BL 15 mL 75 mL 175 mL Buffer KB 22 mL 110 mL 260 mL 10 x Red Blood Cell Lysis Buffer 30 mL 160 mL 400 mL DNA Wash Buffer 12 mL 50 mL 2 x 50 mL Elution Buffer 20 mL 100 mL 250 mL Protease K 12 mg 2x30 mg 5x30 mg RNase A (20 mg/mL) 45 μL 210 μL 520 μL Instruction Booklet 1 1 1 Before Starting The kit is designed to be simple, fast, and reliable provided that all steps are followed diligently. Please read the entire booklet and get all necessary supplies and equipments. Important Materials provided by user Safety Information Buffer BL contains chaotropic salts, which may form reactive compounds when combines with bleach, Do not add bleach or acidic solutions directly to the preparation waste, ware gloves and protective eyewear when handling.
- Model: GD2314-02
- Manufactured by: Biomiga