Description :

Introduction

The EZgene^TM Viral DNA/RNA kit provides an easy and reliable method for isolating total viral DNA/RNA from plasma, serum, whole blood, urine and cell culture supernatant. This procedure has been tested for isolating nucleic acids from Hepatitis A, Hepatitis C and HIV. The isolated DNA/RNA can be used for PCR, RT-PCR and other downstream applications.

Storage and Stability

All other components can be stored at room temperature. All kit components are guaranteed for 1 year from the date of purchasing.

Kit Contents

*Add 8 mL (VR6512-00) or 60 mL (VR6512-01) or 96  mL (VR6512-02) 100% ethanol into each Wash Buffer bottle before use.

Before Starting

Prepare all components and get all necessary materials ready by examining this instruction booklet and become familiar with each steps.

Important

•  Add 1% volume of β-mercaptoethanol to Buffer PLY before use and store at 4 ^oC.
•  Add 8 mL (VR6512-00) or 60 mL (VR6512-01) or 96 mL (VR6512-02) 100% ethanol into each Wash Buffer bottle before use.

Materials supplied by users

•  Tabletop microcentrifuge
•  1.5 mL sterile tubes
•  100% ethanol

Note: Perform all steps including centrifugation at room temperature

Trouble Shooting Guide

Operating Protocol