Description :

Introduction

The EZgene^TM Plant gDNA maxi Kit is designed for efficient recovery of genomic DNA up to 60 kb in size from fresh and dried plant tissue samples.  Up to 2 g of wet tissue (or 500 mg dry tissue) can be processed in one column. The system combines the reversible nucleic acid-binding properties of the matrix with the speed and versatility of spin column technology to eliminate polysaccharides, phenolic compounds, and enzyme inhibitors from plant tissue lysates. Purified DNA is suitable for PCR, restriction digestion, and hybridization techniques. There are no organic extractions thus reducing plastic waste and hands-on time to allow multiple samples to be processed in parallel.

Overview

If using the EZgene^TM Plant gDNA Kit for the first time, please read this booklet to become familiar with the procedures. Samples are homogenized and lysed in a high salt buffer. The DNA is bound to the column while proteins and other impurities are removed by wash buffer. The purified DNA is suitable for downstream applications such as endonuclease digestion, thermal cycle amplification, and hybridization techniques.

Storage and Stability

All components of the EZgene^TM Plant gDNA maxi Kit are stable for at least 12 months from date of purchase when stored at 22 ^oC -25 ^oC. RNase A should be stored at 4 ^oC. During shipment or storage in cool ambient conditions, precipitates may form in Buffer P1 and Buffer P2. It is possible to dissolve such deposits by warming the solution at 37  ^oC, though we have found that they do not interfere with overall performance.

Kit Contents

Before Starting

Prepare all components and get all necessary materials ready by examining this instruction booklet and become familiar with each steps.

Important  

•  Dilute DNA Wash Buffer with absolute ethanol as follows and store at room temperature:

GD2614-00   Add 70 mL absolute (96%-100%) ethanol

GD2614-01   Add 210 mL absolute (96%-100%) ethanol

GD2614-02   Add 2 x 210 mL absolute (96%-100%) ethanol per bottle

•  Choose the most appropriate protocol to follow. Procedures are described for each of dried and fresh (or frozen) specimens.
•  Dissolve deposits in Buffer P1, P2 and P3 by warming the solution at 37 ^oC.

Trouble Shooting Guides