Description :

Introduction

The EZgene^TM Soil gDNA Kit is designed for a rapid and reliable purification of high-quality genomic DNA from various soil samples. Up to 1 gram of soil samples can be processed in less than 1 hour. The system combines the reversible nucleic acid-binding properties of ezBind^TM matrix with a propriety buffer system to eliminate PCR inhibiting compounds such as humic acid from soil samples. Purified DNA is suitable for PCR, restriction digestion, and hybridization techniques. There are no organic extractions thus reducing plastic waste and hands- on time to allow multiple samples to be processed in parallel.

In this procedure, soil sample is homogenized and then treated in a specially formulated buffer that contains detergent. Humic acid, proteins, polysaccharides, and other contaminants are subsequently precipitated after a heat-frozen step. DNA is further purified with a DNA spin-column. Two rapid wash steps remove trace contaminants and pure DNA is eluted in water or low ionic strength Elution Buffer. Purified DNA can be directly used in downstream applications without the need for further purification.

Storage and Stability

All components of the EZgene^TM Soil gDNA Kit should be stored at 22^oC-25^oC. Under these conditions, DNA has successfully been purified and used for PCR after 12 months of storage. During shipment, or storage in cool ambient conditions, precipitates may form in some buffers. It is possible to dissolve such deposits by incubation the solution at 65^oC.

Kit Contents

Buffer BL contains chaotropic salts that may form combustive compound with bleach. Use gloves and protective eyeware when handling this solution.

Before Starting                                                          

Please read the entire booklet to become familiar with the EZgene^TM Soil gDNA Kit protocol.

•  Prepare Buffer LX stock solution by adding 10 μL β-mercaptoethanol per 1 mL Buffer LX before use. Each sample will require 600 μL of this solution.
•  Preheat Buffer LX and Elution Buffer at 65 ^oC. Make sure the crystal in Buffer LX is completely dissolved.
•  Dilute DNA Wash Buffer with absolute ethanol as follows and store at room temperature. Add 8 mL (GD2412-00) or 60 mL (GD2412-01) or 96 mL (GD2412-02) to each DNA Wash Buffer bottle before use.

Trouble Shooting Guide

Limited Use and Warranty

This product is warranted to perform as described in its labeling and in Biomiga's literature when used in accordance with instructions. No other warranties of any kind, express or implied, including, without limitation, implied warranties of merchantability or fitness for a particular purpose, are provided by Biomiga. Biomiga's sole obligation and purchaser's exclusive remedy for breach of this warranty shall be, at the option of Biomiga, to replace the products, Biomiga shall have no liability for any direct, indirect, consequential, or incidental damage arising out of the use, the results of use, or the inability to use it product.

For technical support or learn more product information, please contact us at (858) 603-3219 or visit our website at http://www.biomiga.com/

Operating Protocol