Description :

Introduction

The EZgene^TM Tissue RNA Kit provides an easy and fast method for isolating total RNA from tissues, cultured cells within 30 min. Only trace genomic DNA exists in the purified RNA, which can be eliminated by DNase I treatment (See detail in the protocol) when it is necessary. This kit purifies up to 100 µg of total RNA from eukaryotic cells or animal tissues. The purified RNA is ready for RT-PCR, Northern blotting, polyA+ RNA purification, nuclease protection, and in vitro translation.

Storage and stability

DNase I (optional) should be stored at -20°C. All other components can be stored at room temperature. All kit components are guaranteed for 12 months from the date of purchasing.

Kit contents

*Solutions are not supplied. They could be purchased from Biomiga.

Safety information

Buffer LY contains chaotropic salts, which may form reactive compounds when combines with bleach. Do not add bleach or acidic solutions directly to the preparation waste, wear gloves and protective eyewear when handling.

Before starting

Prepare all components and get all necessary materials ready by examining this instruction booklet and become familiar with each steps.

Important

•  Determine the volume of Buffer LY to be used and add 20 µL of b-mercaptoethanol (b-ME) per 1 mL Buffer LY before use. Buffer LY contains b-ME can be stored at room temperature for up to 1 month.
•  Crystals may form in Buffer LY, dissolve the precipitates at 37 ^oC before use.
•  Add 8 mL (R6311-00) or 80 mL (R6311-01) or 96 mL (R6311-02) 100% ethanol to RNA Wash Buffer before use. The final ethanol is 80% (v/v).
•  Optional: Add 800 µL or 9.6 mL (R6311-01) or 48 mL (R6311-02) 100% ethanol to DNase Stop Buffer before use. The final ethanol is 80% (v/v).

Materials supplied by users

•  Tabletop microcentrifuge and 1.5 mL sterile tubes.
•  Vacuum manifold if use vacuum protocol.
•  100% ethanol.
•  b-mercaptoethanol.
•  Optional: DNase I, DNase Buffer

Note: Perform all steps including centrifugation at room temperature

Trouble Shooting Guide

Operating protocal: