Description : Introduction The EZgeneTM Tissue RNA Kit plus combines the reversible binding properties of HiBind RNA technology with a specially designed buffer system which can effectively remove DNA before RNA isolation. Samples are first lysed and homogenized in a specially designed denaturing buffer, which immediately inhibits the activity of RNase and DNase. The lysate is then passed through a EZgeneTM DNA Clearance column which traps the genomic DNA. The flow-through lysate is then applied to RNA column to bind RNA. After two wash steps, purified RNA is eluted with RNase-free water. The whole protocol can be completed in less than 30 minutes. RNA purified using the DNA/RNA method is ready for applications such as RT-PCR, qPCR, differential display, microarrays, etc All components can be stored at room temperature. All kit components are guaranteed for 12 months from the date of purchasing. Kit contents Catalog# R6321-00 R6321-01 R6321-02 Preps 4 50 250 Buffer HLY 4 mL 40 mL 150 mL Buffer HRB 4 mL 50 mL 150 mL RNA Wash Buffer 5 mL 12 mL 50 mL DEPC-Treated ddH2O 1.0 mL 10 mL 30 mL ezBind RNA Columns 4 50 250 DNA Clearance Column 4 50 250 Collection Tubes 12 150 750 Instruction Manual 1 1 1 Operating protocolStorage and stability
- Model: R6321-00
- Manufactured by: Biomiga