Description :

Introduction

Adeno-assiciated viruses, belong to the replication deficient parvovirus family, are small single-stranded DNA viruses. AAVs are important gene delivery tools, which have been used in gene therapy and RNAi delivery.

Traditionally AAVs are purified by ultracentrifugation using CsCl to separate the virus particles from cellular proteins and media components. 

The CsCl ultracentrifugation procedure is time consuming and limited to the volume of cell lysate to be processed.

The ViraTrapTM AAV maxi purification kit is designed for efficient purification of rAAV (Serotype 2 and DJ) vector transfected cell line. 

Up to 3 x 1012 viral particles can be purified from cell lysate of 6 x T75 flasks. 

Each column can be regenerated for purifying the same rAAV. For optimized viral binding and recovery, each column can be regenerated only once.

Storage and Stability

All components are guaranteed for at least 12 months from the date of purchase when stored as follows: Mini column and desalting tube should be stored at 4 °C, and all other materials at RT (22-25°C).

Safety Considerations

The rAAV infected cell lysate and the purified virus can be potential bio-hazardous material and can be infectious to human and animals. All protocols MUST be performed under at least Bio-Safety level 2 working condition.

Before Starting

Be familiar with each step by reading this menu and prepare all materials for the procedure.

Materials Required But Not Supplied 

• 1.ddH₂O
• 2.PBS Buffer
• 3.Cell Scrapers
• 4.0.45 μm Syringe Filter (Sterilized before use)
• 5.0.22 μm Syringe Filter
• 6.10 mL Syringe

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