Description : Introduction The ViraTrapTM adenovirus mini purification kit is designed for fast and efficient purification of recombinant Adenovirus from adenovirus transfected cell culture supernatant. Up to 1 x1012 viral particles can be purified from cell culture media of 1 to 2 T75 flasks. Traditionally the recombinant adenovirus is purified by ultra centrifugation using CsCl to separate the virus particles from cellular proteins and media components. The CsCl ultracentrifugation procedure is time consuming and limited to the amount of cell lysate to be processed. Each column can be regenerated for purifying the same adenovirus. For optimized viral binding and recovery, each column can be regenerated only once. Before starting Familiar with each step by reading this menu and prepare all materials for the procedure. Kit Components V1160-00 V1160-01 V1160-02 Notes Preps 2 10 20 1 5 10 Store at 4°C Press-On cap 2 5 10 Store at RT Centrifugal filter 1 5 10 Store at RT 15 mL Collection Tube 2 10 20 Store at RT 10x AV Wash Buffer 10 mL 30 mL 60 mL Store at RT 2x AV Elution Buffer 10 mL 30 mL 60 mL Store at RT Regeneration Buffer 10 mL 30 mL 60 mL Store at RT The Guaranteed shelf life is 12 months from the date of purchase. The adenovirus infected cell media and the purified virus can be potential bio-hazardous material and can be infectious to human and animals. All protocols MUST be performed under at least Bio-Safety level 2 working condition. Materials Required But Not Supplied 1. ddH2O 2. PBS 3. 0.45 μm and 0.22 μm filters 4. Rack holder for columns Stability
Safety considerations
- Model: V1160-02
- Manufactured by: Biomiga