L-column ODS-P
CERI L-column ODS-P (C18) Micro Guard Cartridge Trap Column, 5 μm, 5mm Length x 0.3mm ID - 3 per pack
For PEEK-sleeved Micro / Nano Columns
Product Number: 652457
Specifications:
Particle size | 5 μm |
Pore size | 12 nm (120 Å) |
Surface area | 340 m2/g |
Carbon contents | 17% |
Bonded phase | Octadecyl silyl (C18, ODS) |
End-capping | Advanced end-capping |
USP category | L 1 |
Usable pH range | pH 1 - pH 9 |
Connection type: | 1/16-inch Waters (W) |
L-column series is the column for reversed-phase high-performance liquid chromatography (RP-HPLC) that enabled separation only by hydrophobic interaction because of inhibition of the secondary interaction with residual silanol groups and metal impurities.
L-column ODS-P is ideal for the analysis of proteins and peptides. The base silica has a pore diameter of 30 nm. Adsorption is minimized and proteins and peptides elute with sharp peaks. Biological samples are often analyzed using 1 % TFA in the mobile phase and L-column ODS-P is exceptionally stable in strongly acidic mobile phase.
USP
L1: Octadecyl silane chemically bonded to porous or non-porous silica or ceramic micro-particles, 1.5 to 10 μm in diameter, or a monolithic rod.
Trap column
In proteomic analysis using LC/MS/MS, the trap column is indispensable for increasing the injection volume. This trap column retains the target constituents firmly and its media has little irreversible adsorption of the target constituents. Therefore, the actual loss of target constituents is negligible. In the range of 20 - 1000 ng for the insulin B chain, a linear relationship is obtained between the injection volume and the peak area.
A small dead volume, low-adsorption property and high retention ability that traps target constituents are required effectively in a trap column. L-column trap column does not decrease the theoretical plate number because of its very small dead volume. Not only that, because it is a cartridge-type trap column, it is more economical.
- Holder - 652452
Additional CERI Products:
- CERI L-column ODS-P HPLC Columns
- CERI L-Column HPLC Columns
- CERI L-column2 HPLC Columns
- CERI L-column3 HPLC Columns
- Model: 652457
- Manufactured by: CERI