Dr. Maisch Exsil 100 SCX, 5 µm, 100 Å, 100 g bulk Material for HPLC Columns - 5136530.0100

Dr. Maisch Exsil 100 SCX, 5 µm, 100 Å, 100 g bulk Material for HPLC Columns

Product Number: 5136530.0100

Exsil 100, the standard material, is supplied in a range of particle sizes and bonded phases to cover a wide range of applications. Exsil 80 is a higher surface area, complementary range of materials.

SCX technology generally refers to Strong Cation Exchange technology, primarily used in chromatography for separating molecules based on their charge. It involves using a column with a negatively charged surface (typically sulfonic acid groups) to selectively bind positively charged molecules. This technique is widely used in the analysis and purification of biomolecules, particularly proteins and peptides, including monoclonal antibodies.

• Strong Cation Exchange (SCX): A type of ion exchange chromatography where the stationary phase (the column packing material) has a strong negative charge (e.g., sulfonic acid groups).
• Mechanism: Positively charged molecules in a sample bind to the negatively charged column, while neutral or negatively charged molecules pass through.
• Separation: By changing the solution conditions (e.g., pH, ionic strength), the bound molecules can be eluted (released) from the column, allowing for separation.
  Biomolecule Analysis and Purification: SCX is crucial for separating and purifying proteins, peptides, and other charged biomolecules.
• Monoclonal Antibody (mAb) Characterization: It is used to separate and analyse different charge variants of mAbs, which can be indicative of product quality.
• Peptide Mapping: SCX can be combined with other separation techniques (like reversed-phase chromatography) to create comprehensive peptide maps.
• Sample Preparation: SCX solid-phase extraction (SPE) cartridges are used to isolate and purify specific molecules from complex mixtures.
• Exsil 100: Columns with a pore size of 100 Å Angstroms (10 nm)