Dr. Maisch Exsil 80 Amino Analytical HPLC Column, 5 µm, 80 Å, 2 x 50 mm, 1/Pk - 5136488.s0502

Dr. Maisch Exsil 80 Amino Analytical HPLC Column, 5 µm, 80 Å, 2 x 50 mm, 1/Pk

Product Number: 5136488.s0502

Exsil 80

• 80 Å
• 0.51 cc/g
• 226 m2/g 150 m2/ml
• Type A Silica and Bonded Phases
• Alternative to Spherisorb
• Available in 1.5, 3, 5 and 10 μm particle sizes
• Exsil 80: Columns with a pore size of 80 Å Angstroms (8 nm)

The Exsil 80 range has a higher surface area and greater retention than the original Exsil 100 which in some instances provides a better alternative to Spherisorb.

New is the addition of a 1.5μm ODS phase to allow faster separations of current applications. When used with appropriate hardware, efficiencies of over 200,000 plates/m are achievable in 3 to 5 cm columns. The range of phases continues to provide a comprehensive selection for all standard applications.

Amino columns are a type of HPLC (High Performance Liquid Chromatography) column that utilizes a silica-based packing material with aminopropyl groups (-NH2) bonded to it. They are versatile and can be used in various chromatographic modes, including normal phase, reversed phase, and weak anion exchange. Amino columns are particularly well-suited for separating carbohydrates, sugars, and vitamins.

• Versatility: Amino columns can be used in multiple separation modes.
• Normal Phase: Amino columns can be used in normal phase chromatography, similar to silica columns, but with faster gradient changes, according to Analytics-Shop.
• Reversed Phase: They can also be used in reversed phase mode, particularly for separating carbohydrates and sugars, according to UVISON.com.
• Weak Anion Exchange: In some cases, amino columns can act as weak anion exchangers, especially when paired with appropriate buffers and organic modifiers.
• Carbohydrate Analysis: A common application is the separation and analysis of carbohydrates, including mono-, di-, and trisaccharides.
• Retention Mechanisms: The separation mechanism in amino columns is complex, involving a combination of interactions, including hydrophobic, hydrogen bonding, and electrostatic interactions.
• Mobile Phase: Common mobile phases include acetonitrile/water mixtures.
• Temperature: Column temperatures can range from ambient to 35°C for carbohydrate analysis.
• USP Classification: Amino columns are often classified as USP L8.

Specifications: