Hamilton PRP-1 HPLC Columns

PRP-1 Columns are beneficial in a number of ways:

Wide pH Stability
Stability from pH 1 to 13 allows for the analysis of samples at high pH (8-13) without the possibility of column degradation or loss of efficiency. An expanded pH range also allows samples to be chromatographed while ionized. This greatly enhances the detectability of some samples. The chromatogram below illustrates the separation of 3 barbitals on a C18 column at pH 4.5 with detection at 210 nm compared to the separation of barbitals on a PRP-1 column at pH 10.0 with detection at 254 nm. As these applications illustrate, at pH 4.5 the
barbitals are not ionized so their detection must be performed at low UV (190-210 nm) wavelengths. When the separation is done on PRP-1at pH 10.0 the barbitals become ionized and detection at 254 nm is greatly enhanced.