The separation of carbohydrates by anion exchange at basic pH is possible because each carbohydrate carries a different negative charge at basic pH. To utilize the full potential of the RCX-10 column (gradient separations) a Pulsed Amperometric Detector (PAD) is recommended. A PAD allows utilization of both gradient and isocratic elution for the separation of carbohydrates. A typical mobile phase is sodium hydroxide and sodium acetate. When the concentration of the mobile phase is changed, a variety of samples can be separated. Application #98 illustrates the gradient separation of carbohydrates in Jerusalem Artichoke Tubers. Note the wide range of oligomers separated (DP1 to DP17). When used for isocratic separations a conductivity, refractive index, ultraviolet or pulsed amperometric detector can be used to quickly determine mono and disaccharides.
Polymeric anion exchange packing for isocratic or gradient separation of carbohydrates
- - pH stable from 1 to 13
- Isocratic separation of mono and disaccharides
- Gradient separation of oligomers up to DP8
- Compatible with PAD, conductivity and RI detectors
Product Features
- One particle size: 7 µm
- Two column internal diameters: 4.1 and 4.6 mm
- Two column materials: 316 stainless steel and PEEK
- Guard columns in stainless steel and PEEK
