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Imtakt Scherzo SW-C18

World’s first ODS column with Anion and Cation Exchange. Low amount of strong ionic ligands, effective for strong ionic compounds.

Scherzo SW-C18 includes a few amount of strong ionic ligands which is a novel structural idea from
traditional ion-exchange columns. This ssurface structure offers strong ionic compounds elution.

Scherzo SW-C18 has a small amount of strong ionic ligands which is a novel structural idea from traditional ion-exchange columns. This surface structure provides better elution of strong ionic compounds siRNA (small interfering RNA), around 21mer base paired double stranded RNA, is getting lots of attention for next generation medicine. But it is very polar and has a lot of phosphoric acids inside, therefore difficult to analyze in RP mode.

SW-C18 can separate not only siRNA, but also ATP or oligo-nucleotides without adding an ionpairing reagent in the eluent. SW-C18 is applicable for strong ionic compounds like phosphoric compounds because there is a small amount of ionic ligands on the surface of the stationary phase.

Scherzo SW-C18 will provide excellent performance for basic drug compounds and ionic metabolites
between low and neutral pH conditions due to a small amount of strong anionic / cationic ligands.
SW-C18 may be useful for DMPK or metabolome studies.

Separation modes of Scherzo C18 columns

Separation Mode Stationary Phase Properties
Reversed-Phase Octadecyl Increasing organic solvant composition (decreasing polarity of eluent) decreases retention.
Anion Exchange Cation Increasing iconic strength (salt or acid concentration decreases retention for acidic compounds. Generally low pH increases retention.
Cation Exchange Anion Increasing salt concentration decreases retention for basic compounds. SM-C18 retains more with increasing pH, while SS-C18 and SW-C18 retain more at lower pH.
Normal-Phase Anion / Cation Polar solutes, which cannot be retained with 100% aqueous eluent may be retained by using > 50% organic solvent composition due to electrostatic interation.

Elution strategies by Scherzo C18 columns

Non-ionic
hydrophobic solutes
Organic solvent composition should be optimized (similar to conventional ODS).
Peak shape is often improved by using 0.1% acetic acid.
Ionic
hydrophobic solutes
A combination of organic solvent and 20-100mM of salt or acid at optimal pH
should be used for separation of both acidic / basic compounds.
Weak ionic-polar solutes SS-C18 is often the best choice for weak ionic polar solutes. Ionic strength should be
increased for compounds that contain multiple ionic functional groups. Neutral pH
conditions are required for mono-carboxylic acid compounds.
Strong ionic-polar solutes SW-C18 may be useful when it is difficult to elute on SS-C18 or SM-C18. Strong
ionic compounds have strong ionic interaction, so it is recommended to use multiple
gradient elutions with ionic strength and organic solvent composition.

 



Item Name Product Image Price

Imtakt Scherzo SW-C18 1.5mm ID, 100mm Length, 3um,multi-mode ODS (RP+Anion+Cation) - SW074

Scherzo SW-C18  multi-mode ODS HPLC Column Scherzo SW-C18 Reversed Phase (RP) + Anion Exchange (AX) + Cation Exchange (CX) Multi-Mode ODS Analytical HPLC Column Imtakt Part Number: SW074 Column Specifications ID: 1.5 mm ...
Imtakt Scherzo SW-C18 1.5mm ID, 100mm Length, 3um,multi-mode ODS (RP+Anion+Cation) - SW074 £378.77Buy Now

Imtakt Scherzo SW-C18 1.5mm ID, 150mm Length, 3um,multi-mode ODS (RP+Anion+Cation) - SW075

Scherzo SW-C18 1.5 ID (mm) 150 L (mm) 3um,multi-mode ODS (RP+Anion+Cation)
Imtakt Scherzo SW-C18 1.5mm ID, 150mm Length, 3um,multi-mode ODS (RP+Anion+Cation) - SW075 £414.51Buy Now

Imtakt Scherzo SW-C18 1.5mm ID, 250mm Length, 3um,multi-mode ODS (RP+Anion+Cation) - SW076

Scherzo SW-C18 1.5 ID (mm) 250 L (mm) 3um,multi-mode ODS (RP+Anion+Cation)
Imtakt Scherzo SW-C18 1.5mm ID, 250mm Length, 3um,multi-mode ODS (RP+Anion+Cation) - SW076 £536.00Buy Now

Imtakt Scherzo SW-C18 1.5mm ID, 30mm Length, 3um,multi-mode ODS (RP+Anion+Cation) - SW071

Scherzo SW-C18 1.5 ID (mm) 30 L (mm) 3um,multi-mode ODS (RP+Anion+Cation)
Imtakt Scherzo SW-C18 1.5mm ID, 30mm Length, 3um,multi-mode ODS (RP+Anion+Cation) - SW071 £321.60Buy Now

Imtakt Scherzo SW-C18 1.5mm ID, 50mm Length, 3um,multi-mode ODS (RP+Anion+Cation) - SW072

Scherzo SW-C18 1.5 ID (mm) 50 L (mm) 3um,multi-mode ODS (RP+Anion+Cation)
Imtakt Scherzo SW-C18 1.5mm ID, 50mm Length, 3um,multi-mode ODS (RP+Anion+Cation) - SW072 £321.60Buy Now

Imtakt Scherzo SW-C18 1.5mm ID, 75mm Length, 3um,multi-mode ODS (RP+Anion+Cation) - SW073

Scherzo SW-C18 1.5 ID (mm) 75 L (mm) 3um,multi-mode ODS (RP+Anion+Cation)
Imtakt Scherzo SW-C18 1.5mm ID, 75mm Length, 3um,multi-mode ODS (RP+Anion+Cation) - SW073 £343.04Buy Now

Imtakt Scherzo SW-C18 10mm ID, 100mm Length, 3um,multi-mode ODS (RP+Anion+Cation) - SW0P4

Scherzo SW-C18 10 ID (mm) 100 L (mm) 3um,multi-mode ODS (RP+Anion+Cation)
Imtakt Scherzo SW-C18 10mm ID, 100mm Length, 3um,multi-mode ODS (RP+Anion+Cation) - SW0P4 £857.60Buy Now

Imtakt Scherzo SW-C18 10mm ID, 150mm Length, 3um,multi-mode ODS (RP+Anion+Cation) - SW0P5

Scherzo SW-C18 10 ID (mm) 150 L (mm) 3um,multi-mode ODS (RP+Anion+Cation)
Imtakt Scherzo SW-C18 10mm ID, 150mm Length, 3um,multi-mode ODS (RP+Anion+Cation) - SW0P5 £1,072.00Buy Now

Imtakt Scherzo SW-C18 10mm ID, 250mm Length, 3um,multi-mode ODS (RP+Anion+Cation) - SW0P6

Scherzo SW-C18 10 ID (mm) 250 L (mm) 3um,multi-mode ODS (RP+Anion+Cation)
Imtakt Scherzo SW-C18 10mm ID, 250mm Length, 3um,multi-mode ODS (RP+Anion+Cation) - SW0P6 £1,286.40Buy Now

Imtakt Scherzo SW-C18 10mm ID, 30mm Length, 3um,multi-mode ODS (RP+Anion+Cation) - SW0P1

Scherzo SW-C18 10 ID (mm) 30 L (mm) 3um,multi-mode ODS (RP+Anion+Cation)
Imtakt Scherzo SW-C18 10mm ID, 30mm Length, 3um,multi-mode ODS (RP+Anion+Cation) - SW0P1 £485.97Buy Now

Imtakt Scherzo SW-C18 10mm ID, 50mm Length, 3um,multi-mode ODS (RP+Anion+Cation) - SW0P2

Scherzo SW-C18 10 ID (mm) 50 L (mm) 3um,multi-mode ODS (RP+Anion+Cation)
Imtakt Scherzo SW-C18 10mm ID, 50mm Length, 3um,multi-mode ODS (RP+Anion+Cation) - SW0P2 £571.73Buy Now

Imtakt Scherzo SW-C18 10mm ID, 75mm Length, 3um,multi-mode ODS (RP+Anion+Cation) - SW0P3

Scherzo SW-C18 10 ID (mm) 75 L (mm) 3um,multi-mode ODS (RP+Anion+Cation)
Imtakt Scherzo SW-C18 10mm ID, 75mm Length, 3um,multi-mode ODS (RP+Anion+Cation) - SW0P3 £678.93Buy Now

Imtakt Scherzo SW-C18 1mm ID, 100mm Length, 3um,multi-mode ODS (RP+Anion+Cation) - SW014

Scherzo SW-C18 1 ID (mm) 100 L (mm) 3um,multi-mode ODS (RP+Anion+Cation)
Imtakt Scherzo SW-C18 1mm ID, 100mm Length, 3um,multi-mode ODS (RP+Anion+Cation) - SW014 £378.77Buy Now

Imtakt Scherzo SW-C18 1mm ID, 150mm Length, 3um,multi-mode ODS (RP+Anion+Cation) - SW015

Scherzo SW-C18 1 ID (mm) 150 L (mm) 3um,multi-mode ODS (RP+Anion+Cation)
Imtakt Scherzo SW-C18 1mm ID, 150mm Length, 3um,multi-mode ODS (RP+Anion+Cation) - SW015 £414.51Buy Now

Imtakt Scherzo SW-C18 1mm ID, 250mm Length, 3um,multi-mode ODS (RP+Anion+Cation) - SW016

Scherzo SW-C18 1 ID (mm) 250 L (mm) 3um,multi-mode ODS (RP+Anion+Cation)
Imtakt Scherzo SW-C18 1mm ID, 250mm Length, 3um,multi-mode ODS (RP+Anion+Cation) - SW016 £536.00Buy Now

Imtakt Scherzo SW-C18 1mm ID, 30mm Length, 3um,multi-mode ODS (RP+Anion+Cation) - SW011

Scherzo SW-C18 1 ID (mm) 30 L (mm) 3um,multi-mode ODS (RP+Anion+Cation)
Imtakt Scherzo SW-C18 1mm ID, 30mm Length, 3um,multi-mode ODS (RP+Anion+Cation) - SW011 £321.60Buy Now

Imtakt Scherzo SW-C18 1mm ID, 50mm Length, 3um,multi-mode ODS (RP+Anion+Cation) - SW012

Scherzo SW-C18 1 ID (mm) 50 L (mm) 3um,multi-mode ODS (RP+Anion+Cation)
Imtakt Scherzo SW-C18 1mm ID, 50mm Length, 3um,multi-mode ODS (RP+Anion+Cation) - SW012 £321.60Buy Now

Imtakt Scherzo SW-C18 1mm ID, 75mm Length, 3um,multi-mode ODS (RP+Anion+Cation) - SW013

Scherzo SW-C18 1 ID (mm) 75 L (mm) 3um,multi-mode ODS (RP+Anion+Cation)
Imtakt Scherzo SW-C18 1mm ID, 75mm Length, 3um,multi-mode ODS (RP+Anion+Cation) - SW013 £343.04Buy Now

Imtakt Scherzo SW-C18 2mm ID, 100mm Length, 3um,multi-mode ODS (RP+Anion+Cation) - SW024

Scherzo SW-C18 2 ID (mm) 100 L (mm) 3um,multi-mode ODS (RP+Anion+Cation)
Imtakt Scherzo SW-C18 2mm ID, 100mm Length, 3um,multi-mode ODS (RP+Anion+Cation) - SW024 £378.77Buy Now

Imtakt Scherzo SW-C18 2mm ID, 10mm Length, 3um,multi-mode ODS (RP+Anion+Cation) - SW020

Scherzo SW-C18 2 ID (mm) 10 L (mm) 3um,multi-mode ODS (RP+Anion+Cation)
Imtakt Scherzo SW-C18 2mm ID, 10mm Length, 3um,multi-mode ODS (RP+Anion+Cation) - SW020 £321.60Buy Now