Miles Scientific Analtech Brand Silica Gel HF (Binder-free adsorbent, Fluorescent indicator UV254) TLC Plates, with 5% Ammonium Sulphate, 250 um, 5 x 20 cm, 25/Pk - P75031

Glass-backed Analytical TLC Plates

Miles Scientific Analtech Brand Silica Gel HF (Binder-free adsorbent, Fluorescent indicator UV254) TLC Plates, with 5% Ammonium Sulfate, 250µm, 5x20cm, 25Pk

Product Number: P75031

Weight: 3.10 LBS

H - Binder-free adsorbent

F - Fluorescent indicator UV254. (Can be added to any adsorbent.)

Ammonium Sulfate (5%) Modified Silica Gel H & HF UNIPLATES™ (Self-Charring)

A common method for visualizing organic compounds on a silica gel TLC plate is charring. This is usually accomplished by spraying the plate with dilute sulfuric acid followed by heating. Organic substances appear as brown to black spots against a white background. This method is comparable in sensitivity to the visualization of UVabsorbing compounds against a fluorescent background.

Silica Gel H UNIPLATES containing ammonium sulfate eliminate the need to use messy sulfuric acid for charring. Just evaporate the developing solvent until the plate is completely dry, then heat at 150°-200°C for 30 to 60 minutes to achieve charring. The Analtech VPF chamber is specifically designed for this application. Other percentages of ammonium sulfate may be specified.

Silica Gel H & HF UNIPLATES™ – Binder Free

Silica Gel H & HF UNIPLATES contain no calcium sulfate binder. The adsorbent contains a small amount of colloidal silica which aids in the adherence to the glass plate.

Analtech Brand Silica Gel H / HF TLC Plates

• Silica Pore: 60Å
• Silica Particle Size: 19 μm average
• Binder: none
• Hardness: 1-8

Acceptable Solvents:

All typical chromatography solvents (20% water max)

Visualization Techniques:

Compatible with essentially all visualization reagents, even sulfuric acid charring. Spray reagent only. Dipping not recommended.

General Description:

Very soft surface as no binder is used to adhere the layer to the glass backing. Requires extra caution during use to prevent damage to the layer, which would affect chromatography. However, it is sometimes required when the separation being run is sensitive to any materials other than silica gel in the chromatographic process. Compatible with most mobile phases except water in excess of about 20%. Dip visualization procedures not recommended, but plates do tolerate gentle spray techniques.