Miles Scientific Analtech Silica Gel GHL (Gypsum, Hard Layer inorganic binder) TLC Plates, 250 um, 20 x 20 cm, Prep-scored, with Preadsorbent Zone, 25/Pk - P41001

Glass-backed Analytical TLC Plates

Miles Scientific Analtech Brand Silica Gel GHL (Hard Layer inorganic binder plus Calcium Sulfate, Gypsum) TLC Plates, 250µm, 20x20cm, Prep-scored, with Preadsorbent Zone, 25Pk

Product Number: P41001

Weight: 11.10 LBS

GHL - Hard Layer inorganic binder plus Calcium Sulfate (Gypsum)

Hard Layer Silica Gel GHL & GHLF UNIPLATES™ (Inorganic Binder)

These plates are 100% H20 resistant and are compatible with aqueous developing solvents. They are less rugged than the plates with organic binder but still provide improved handling and sample application compared to Silica Gel G UNIPLATES and are tough enough to write on with a soft pencil, if required.

Hard Layer UNIPLATES with inorganic binder are recommended for all applications including those where visualization occurs through strong charring procedures or in any case in which reagent interaction with an organic binder occurs.

• Layers tolerate 100% water.
• Contains no organic materials.
• Suitable for aggressive visualization procedures.
• Standard scoring for 20x20 cm plates yields four 5x20 cm plates.
• Channeled 20x20 cm plates have 19 channels.
• One box of scored 10x20 cm plates yields two hundred 2.5x10 cm plates. 

The preadsorbent zone on HPTLC UNIPLATES is 1.5 cm high. Low-form plates have the preadsorbent zone along the 20 cm edge. High-form plates have the preadsorbent zone along the 10 cm edge.

Analtech Brand Silica Gel GHL / GHLF

• Silica Pore: 60Å
• Silica Particle Size: 8-10 μm average
• Binder: Calcium sulfate a.k.a. gypsum (inorganic) with proprietary
• hardening agent (inorganic)
• Hardness: 80-160 (can write on gently with pencil)

Acceptable Solvents:

All typical chromatography solvents (even 100% water)

Visualization Techniques:

Compatible with essentially all visualization reagents, even sulfuric acid charring. Reagent may be dipped or sprayed.

General Description:

Moderate hardness layer combines rugged surface characteristics with freedom to use essentially any mobile phase component or visualization reagent. Binder is compatible from nonpolar hexane all the way through the eluotropic series to 100% aqueous systems. Being inorganic, the binder allows use of aggressive visualization systems such as sulfuric acid charring and dipping procedures.

Preadsorbent UNIPLATES™

• 3cm wide sample application zone
• Concentrates sample spots on the plate
• Simplifies sample preparation and application
• Channeled version available

Preadsorbent UNIPLATES have an inert spotting zone along the bottom edge of the plate. This sample application region serves as a “holding zone” for sample spots until development of the plate is initiated. There is no significant resolution of sample components by the stationary phase in this region. All soluble components migrate with the solvent front, concentrating diffuse sample spots into vertically narrow bands.

The adsorbent zone extends from the upper edge of the preadsorbent zone and covers the remaining area of the TLC plate. This zone exhibits the selective retention of sample components characteristic of the adsorbent employed. Separation of sample components occurs in the adsorbent zone in the same way as on a standard TLC plate.

Preconcentration of the sample results in compact zones at the beginning of the separation. Also, resolution and sensitivity are less dependent on sample application technique since all samples are concentrated to narrow bands regardless of the size or position in the zone of the sample spots.

Prep-Scored UNIPLATES™

Prep-Scored UNIPLATES are designed especially for preparative TLC procedures where destructive visualization techniques must be used to locate separated zones.

Prep-Scored UNIPLATES are scored 2.5 cm from each vertical edge. After developing, the 2.5 cm strips between each score mark and the edge can be easily “snapped” away from the center portion of the plate.

Visualization of separated zones on these 2.5 cm strips followed by realignment with the center portion provides a convenient method for locating zones to be recovered by scraping.