Miles Scientific Analtech Silica Gel GHLF (Gypsum, Hard Layer inorganic binder, Fluorescent indicator) TLC Plates, 250 um, 20 x 20 cm, Scored, Preadsorbent Zone, 25/Pk - P42311

Glass-backed Analytical TLC Plates

Miles Scientific Analtech Brand Silica Gel GHLF (Hard Layer, Gypsum, Fluorescent indicator) TLC Plates, 250µm, 20x20cm, scored, with Preadsorbent Zone, 25Pk 

Product Number: P42311

Weight: 11.10 LBS

GHL - Hard Layer inorganic binder plus Calcium Sulfate (Gypsum)

F - Fluorescent indicator UV254. (Can be added to any adsorbent.)

Hard Layer Silica Gel GHL & GHLF UNIPLATES™ (Inorganic Binder)

These plates are 100% H20 resistant and are compatible with aqueous developing solvents. They are less rugged than the plates with organic binder but still provide improved handling and sample application compared to Silica Gel G UNIPLATES and are tough enough to write on with a soft pencil, if required.

Hard Layer UNIPLATES with inorganic binder are recommended for all applications including those where visualization occurs through strong charring procedures or in any case in which reagent interaction with an organic binder occurs.

• Layers tolerate 100% water.
• Contains no organic materials.
• Suitable for aggressive visualization procedures.
• Standard scoring for 20x20 cm plates yields four 5x20 cm plates.
• Channeled 20x20 cm plates have 19 channels.
• One box of scored 10x20 cm plates yields two hundred 2.5x10 cm plates. 

The preadsorbent zone on HPTLC UNIPLATES is 1.5 cm high. Low-form plates have the preadsorbent zone along the 20 cm edge. High-form plates have the preadsorbent zone along the 10 cm edge.

Analtech Brand Silica Gel GHL / GHLF

• Silica Pore: 60Å
• Silica Particle Size: 8-10 μm average
• Binder: Calcium sulfate a.k.a. gypsum (inorganic) with proprietary
• hardening agent (inorganic)
• Hardness: 80-160 (can write on gently with pencil)

Acceptable Solvents:

All typical chromatography solvents (even 100% water)

Visualization Techniques:

Compatible with essentially all visualization reagents, even sulfuric acid charring. Reagent may be dipped or sprayed.

General Description:

Moderate hardness layer combines rugged surface characteristics with freedom to use essentially any mobile phase component or visualization reagent. Binder is compatible from nonpolar hexane all the way through the eluotropic series to 100% aqueous systems. Being inorganic, the binder allows use of aggressive visualization systems such as sulfuric acid charring and dipping procedures.

Preadsorbent UNIPLATES™

• 3cm wide sample application zone
• Concentrates sample spots on the plate
• Simplifies sample preparation and application
• Channeled version available

Preadsorbent UNIPLATES have an inert spotting zone along the bottom edge of the plate. This sample application region serves as a “holding zone” for sample spots until development of the plate is initiated. There is no significant resolution of sample components by the stationary phase in this region. All soluble components migrate with the solvent front, concentrating diffuse sample spots into vertically narrow bands.

The adsorbent zone extends from the upper edge of the preadsorbent zone and covers the remaining area of the TLC plate. This zone exhibits the selective retention of sample components characteristic of the adsorbent employed. Separation of sample components occurs in the adsorbent zone in the same way as on a standard TLC plate.

Preconcentration of the sample results in compact zones at the beginning of the separation. Also, resolution and sensitivity are less dependent on sample application technique since all samples are concentrated to narrow bands regardless of the size or position in the zone of the sample spots.

Scored UNIPLATES™

Most UNIPLATES can be supplied with the glass backing scored at specific locations to permit convenient “snapping” into smaller plates. This Analtech feature provides increased versatility and can mean cost savings over plates already cut to smaller sizes.

Different adsorbents, mobile phases, and analytical conditions can be tested quickly and economically. A plate can be spotted, then snapped into several smaller plates for developing in different solvent mixtures to determine the best separation.

A plate can be spotted, developed, then snapped into several smaller plates, and then subjected to different visualization techniques.

Scored UNIPLATES are available in two standard formats:

1) 20 x 20 cm scored UNIPLATES have three score marks 5 cm apart. Each plate can be snapped to produce four 5 x 20 cm plates or any multiple of 5 cm width.

2) 10 x 20 cm scored UNIPLATES have seven score marks 2.5 cm apart. Each can be snapped to produce eight 2.5 x 10 cm plates or any multiple of 2.5 cm width.