Miles Scientific Analtech Silica Gel HLF (Hard Layer organic binder, Fluorescent indicator) TLC Plates, 250 um, 20x20cm, Scored (2.5x20cm), Preadsorbent Zone, 25/Pk - P44311

Glass-backed Analytical TLC Plates

Miles Scientific Analtech Brand Silica Gel HLF (Hard Layer organic binder, Fluorescent indicator UV254) TLC Plates, 250µm, 20x20cm, scored (2.5x20cm), with Preadsorbent Zone, 25Pk

Product Number: P44311

Weight: 11.10 LBS

HL - Hard Layer organic binder

F - Fluorescent indicator UV254. (Can be added to any adsorbent.)

Analtech Brand Silica Gel HL / HLF TLC Plates

• Silica Pore: 60Å
• Silica Particle Size: 8-10 μm average
• Binder: Proprietary polymer (organic)
• Hardness: 200-400 (easy to write on with pencil)

Acceptable Solvents:

All typical chromatography solvents (80% water max)

Visualization Techniques:

Compatible with most visualization reagents except sulfuric acid charring. Reagent may be dipped or sprayed. Some incompatibility with certain acidic reagents.

General Description:

Best layer choice unless contra-indicated by noted incompatibilities of visualization reagents or mobile phase composition. Hard layer organic binder system is the most rugged, providing easy handling of layer during sample application, mobile phase development, visualization and data interpretation. Rugged layer surface provides consistent results independent of operator technique. Quick development times and uniform visualization properties provide lot-to-lot consistency and reduce seasonal variability in chromatography.

Hard Layer Silica Gel HL & HLF UNIPLATES™ (Organic Binder)

These are the most rugged silica gel plates available. They not only ease handling and sample application, but also permit the use of up to 80% water in the developing solvent without loss of adherence of the adsorbent layer to the glass plate. Organic binder plates are recommended for all TLC applications except those that use vigorous charring for visualization. Virtually all other visualization methods are free from interference from the organic binder.

Hard Layer Silica Gel UNIPLATES represent an advancement in thin layer adsorbent technology. Special high performance silica gel with small average diameter and narrow particle size distribution results in separations 25% to 30% faster than with most other TLC plates. Separated spots are more compact and better resolved, which allows visualization at lower sample levels. The enhanced abrasion resistance means easier handling and sample application because plate surface gouging is virtually eliminated. Analtech Hard Layer UNIPLATES are available with either organic or inorganic binders. Binder choice has little effect on the adsorptive properties of the adsorbent layer.

Preadsorbent UNIPLATES™

• 3cm wide sample application zone
• Concentrates sample spots on the plate
• Simplifies sample preparation and application
• Channeled version available

Preadsorbent UNIPLATES have an inert spotting zone along the bottom edge of the plate. This sample application region serves as a “holding zone” for sample spots until development of the plate is initiated. There is no significant resolution of sample components by the stationary phase in this region. All soluble components migrate with the solvent front, concentrating diffuse sample spots into vertically narrow bands.

The adsorbent zone extends from the upper edge of the preadsorbent zone and covers the remaining area of the TLC plate. This zone exhibits the selective retention of sample components characteristic of the adsorbent employed. Separation of sample components occurs in the adsorbent zone in the same way as on a standard TLC plate.

Preconcentration of the sample results in compact zones at the beginning of the separation. Also, resolution and sensitivity are less dependent on sample application technique since all samples are concentrated to narrow bands regardless of the size or position in the zone of the sample spots.

Scored UNIPLATES™

Most UNIPLATES can be supplied with the glass backing scored at specific locations to permit convenient “snapping” into smaller plates. This Analtech feature provides increased versatility and can mean cost savings over plates already cut to smaller sizes.

Different adsorbents, mobile phases, and analytical conditions can be tested quickly and economically. A plate can be spotted, then snapped into several smaller plates for developing in different solvent mixtures to determine the best separation.

A plate can be spotted, developed, then snapped into several smaller plates, and then subjected to different visualization techniques.

Scored UNIPLATES are available in two standard formats:

1) 20 x 20 cm scored UNIPLATES have three score marks 5 cm apart. Each plate can be snapped to produce four 5 x 20 cm plates or any multiple of 5 cm width.

2) 10 x 20 cm scored UNIPLATES have seven score marks 2.5 cm apart. Each can be snapped to produce eight 2.5 x 10 cm plates or any multiple of 2.5 cm width.