Miles Scientific Analtech Silica Gel RP18F (Octadecyl Bonded, Reversed Phase, Fluorescent indicator) Preadsorbent TLC Plates, 200 um, 20 x 20 cm, 25/Pk - P91011

Glass-backed Analytical TLC

Miles Scientific Analtech Brand Silica Gel RP18F (Octadecyl Bonded, Reversed Phase, Fluorescent indicator) Preadsorbent TLC Plates, 200µm, 20x20cm, 25Pk

Product Number: P91011

Weight: 12.10 LBS

RP18 - Octadecyl Bonded Silica Gel Reversed Phase for HPTLC

F - Fluorescent indicator UV254. (Can be added to any adsorbent.)

Reversed Phase Octadecyl Silanized Silica Gel

These reversed phase TLC plates have been manufactured to be greater than 50% and less than 100% silanized. Because of this they are hydrophobic, but wettable (unlike part numbers 164077 & 165077 which are 100% silanized).

Features

• 14% carbon content 
• 20x20 cm glass backing
• 250um coating thickness
• 25 plates/box 

Recomended Applications

aminophenols, barbiturates, preservatives, nucleobases, polycyclic aromatic hydrocarbons, steroids, tetracyclines, plasticizers (phthalates)

Preadsorbent UNIPLATES™

• 3cm wide sample application zone
• Concentrates sample spots on the plate
• Simplifies sample preparation and application
• Channeled version available

Preadsorbent UNIPLATES have an inert spotting zone along the bottom edge of the plate. This sample application region serves as a “holding zone” for sample spots until development of the plate is initiated. There is no significant resolution of sample components by the stationary phase in this region. All soluble components migrate with the solvent front, concentrating diffuse sample spots into vertically narrow bands.

The adsorbent zone extends from the upper edge of the preadsorbent zone and covers the remaining area of the TLC plate. This zone exhibits the selective retention of sample components characteristic of the adsorbent employed. Separation of sample components occurs in the adsorbent zone in the same way as on a standard TLC plate.

Preconcentration of the sample results in compact zones at the beginning of the separation. Also, resolution and sensitivity are less dependent on sample application technique since all samples are concentrated to narrow bands regardless of the size or position in the zone of the sample spots.

RPS - Reversed Phase Separation

Analtech Reversed Phase Separation (RPS) UNIPLATES are a hydrocarbon impregnated silica gel. The hydrocarbon is adsorbed onto the silica gel surface and is not chemically anchored to the particle. The impregnated RPS UNIPLATE offers several advantages over the bonded RP18 type TLC plates along with some limitations.

In the High Performance format, Analtech offers several varieties of supports. A hydrocarbon impregnated layer, similar to the analytical RPS UNIPLATE, offers complete aqueous solvent compatibility. Three nonpolar bonded supports in the UNIBOND™ series are available: RP2, RP8, and RP18.

These fully silanized silica gels share complete compatibility with all organic solvents. However, due to the extreme hydrophobic nature of the RP18 adsorbent, aqueous wettability is limited to approximately 60% water in the mobile phase. Wettability is correspondingly higher with the slightly more polar shorter chain RP series plates.

The main advantages of the hydrocarbon impregnated Reversed Phase plate are compatibility with aqueous solutions and parallel behavior to most C18 HPLC packings. The RPS UNIPLATE will accept 100% water either as the mobile phase or spotting solvent. This is a great advantage when working with polar sample components such as proteins and peptides.

The RPS impregnated UNIPLATE approximates the adsorption characteristics of the bonded C18 modified silica gels. Therefore, RPS Reversed Phase UNIPLATES are an excellent medium for rapid, inexpensive screening of chromatographic conditions in HPLC method development. Mixtures of water or buffers plus a polar organic modifier (eg., Methanol, acetonitrile, THF, etc.) are easily scouted with RPS UNIPLATES. However, since the hydrocarbon is adsorbed but not chemically anchored to the silica gel, the RPS UNIPLATES cannot tolerate nonpolar organic solvents. Ethyl Acetate or less polar organic solvents will strip the hydrocarbon from the silica gel support.