Miles Scientific Analtech Unibond Silica Gel Amino F (Aminopropyl NH2 Bonded, NH2, Fluorescent indicator) HPTLC UNIPLATES, Glass, 150 um, 10 x 10 cm, 25/Pk - P22077

Glass-backed HPTLC Plates

UNIBOND(TM) Amino F HPTLC 150µm 10x10cm (25 plates/box)

25 Plates/Box, 150 μm Thickness, 8μm particle, 60Å pore

Miles Scientific Product Number: P22077

Weight: 3.10 LBS

• NH2 - Aminopropyl Bonded Silica Gel
• F - Fluorescent indicator UV254. (Can be added to any adsorbent.)
• Fluorescent indicator has green fluorescence at 254 nm
• HPTLC - High Performance Thin Layer Chromatography

Normal Phase HPTLC UNIPLATES - Unibond™ Amino (NH2) & Cyano (CN) HPTLC UNIPLATES™

• High efficiency 8 micron (ave) adsorbent with narrow particle and pore size distribution
• 150 micron adsorbent layer thickness for fast separations
• Homogeneous layer allows linear or radial separations
• Smooth adsorbent surface for noise free densitometry

High Performance UNIPLATES provide high resolution separations in less than five minutes with migration distances as little as 5 to 7 cm. These plates are prepared with high efficiency silica gel having an 8 micron average particle diameter and narrow particle size distribution. The adsorbent layers are only 150 microns thick, resulting in more rapid solvent migration. 

The adsorbent capacity is more than adequate for small samples used in high performance TLC techniques. Typically, sample volumes for high performance TLC are less than one microliter. Sample spots are normally smaller than one millimeter in diameter.

Samples are spotted 1.0 to 1.5 cm from the bottom edge of the plate and 0.3 to 0.5 cm apart. Since smaller samples are applied, one 10 x 10 cm plate can separate as many samples as can be separated on a 20 x 20 cm plate using standard TLC techniques. The smooth homogeneous surface of HPTLC UNIPLATES gives high signal-to-noise ratios for densitometric scanning, thus increasing sensitivity and precision.

The preadsorbent zone on HPTLC UNIPLATES is 1.5 cm high. Low-form plates have the preadsorbent zone along the 20 cm edge. High-form plates have the preadsorbent zone along the 10 cm edge.