Glass-backed HPTLC Plates
Miles Scientific HPTLC-Bonded Phase Sample Pack 150µm 10x20cm scored (5 plates each RP2, RP8, RP18, CN, NH2)
25 Plates/Box, 150 μm Thickness, 8μm particle, 60Å pore
Product Number: P07527
Weight: 7.10 LBS
This Sample Pack Consists of 5 plates each RP2F, RP8F, RP18F, CNF, NH2F (Organic binder)
- RP2 - Ethyl Bonded Silica Gel Reversed Phase for HPTLC
- RP8 - Octyl Bonded Silica Gel Reversed Phase for HPTLC
- RP18 - Octadecyl Bonded Silica Gel Reversed Phase for HPTLC
- CN - Cyanoethyl Bonded Silica Gel
- NH2 - Aminopropyl Bonded Silica Gel
- F - Fluorescent indicator UV254. (Can be added to any adsorbent.)
- HPTLC - High Performance Thin Layer Chromatography
Normal Phase - Unibond™ Amino (NH2) & Cyano (CN) HPTLC UNIPLATES™
- High efficiency 8 micron (ave) adsorbent with narrow particle and pore size distribution
- 150 micron adsorbent layer thickness for fast separations
- Homogeneous layer allows linear or radial separations
- Smooth adsorbent surface for noise free densitometry
High Performance UNIPLATES provide high resolution separations in less than five minutes with migration distances as little as 5 to 7 cm. These plates are prepared with high efficiency silica gel having an 8 micron average particle diameter and narrow particle size distribution. The adsorbent layers are only 150 microns thick, resulting in more rapid solvent migration.
The adsorbent capacity is more than adequate for small samples used in high performance TLC techniques. Typically, sample volumes for high performance TLC are less than one microliter. Sample spots are normally smaller than one millimeter in diameter.
Samples are spotted 1.0 to 1.5 cm from the bottom edge of the plate and 0.3 to 0.5 cm apart. Since smaller samples are applied, one 10 x 10 cm plate can separate as many samples as can be separated on a 20 x 20 cm plate using standard TLC techniques. The smooth homogeneous surface of HPTLC UNIPLATES gives high signal-to-noise ratios for densitometric scanning, thus increasing sensitivity and precision.
The preadsorbent zone on HPTLC UNIPLATES is 1.5 cm high. Low-form plates have the preadsorbent zone along the 20 cm edge. High-form plates have the preadsorbent zone along the 10 cm edge.
Reversed Phase Unibond™ Series HPTLC UNIPLATES™
The availability of both normal phase and Reversed Phase HPTLC UNIPLATES™ completes HPTLC as a
sensible, efficient and cost effective alternative technique for quantitative analysis. These Reversed Phase adsorbents share the features and benefits of silica gel
HPTLC plates: high efficiency adsorbents, 150 μm layer thickness, and smooth surface for densitometry. In the High Performance format, Analtech offers several varieties of supports. A hydrocarbon impregnated layer, similar to the analytical RPS UNIPLATE, offers complete aqueous solvent compatibility Three nonpolar bonded supports in the UNIBOND™ series are available: RP2, RP8, and RP18.
These fully silanized silica gels share complete compatibility with all organic solvents. However, due to the extreme hydrophobic nature of the RP18 adsorbent, aqueous wettability is limited to approximately 60% water in the mobile phase. Wettability is correspondingly higher with the slightly more polar shorter chain RP series plates.
- Model: P07527
- Manufactured by: Miles Scientific