Miles Scientific Analtech HPTLC-GHLF (Gypsum, Hard Layer, Fluorescent indicator UV254) TLC Plates, Glass-backed, 150 um, 10 x 20 cm, 25/Pk - P57027

Glass-backed HPTLC Plates

Miles Scientific Analtech Brand HPTLC-GHLF Thin Layer Chromatography (TLC) Plates (with Fluorescent indicator UV254) - 150µm thickness - 10x20cm - 25 plates per box - 15μm particle, 60Å pore

Product Number: P57027

Weight: 7.10 LBS

• HPTLC - High Performance Thin Layer Chromatography
• G - (Gypsum) Calcium Sulfate Hemihydrate binder
• HL - Hard Layer organic binder
• F - Fluorescent indicator UV254. (Can be added to any adsorbent.)

Normal Phase HPTLC

• High efficiency 8 micron (ave) adsorbent with narrow particle and pore size distribution
• 150 micron adsorbent layer thickness for fast separations
• Homogeneous layer allows linear or radial separations
• Smooth adsorbent surface for noise free densitometry

High Performance UNIPLATES provide high resolution separations in less than five minutes with migration distances as little as 5 to 7 cm. These plates are prepared with high efficiency silica gel having an 8 micron average particle diameter and narrow particle size distribution. The adsorbent layers are only 150 microns thick, resulting in more rapid solvent migration. The adsorbent capacity is more than adequate for small samples used in high performance TLC techniques. Typically, sample volumes for high performance TLC are less than one microliter. Sample spots are normally smaller than one millimeter in diameter.

Samples are spotted 1.0 to 1.5 cm from the bottom edge of the plate and 0.3 to 0.5 cm apart. Since smaller samples are applied, one 10 x 10 cm plate can separate as many samples as can be separated on a 20 x 20 cm plate using standard TLC techniques. The smooth homogeneous surface of HPTLC UNIPLATES gives high signal-to-noise ratios for densitometric scanning, thus increasing sensitivity and precision.

Hard Layer Silica Gel GHL & GHLF UNIPLATES™ (Inorganic Binder)

These plates are 100% H20 resistant and are compatible with aqueous developing solvents. They are less rugged than the plates with organic binder but still provide improved handling and sample application compared to Silica Gel G UNIPLATES and are tough enough to write on with a soft pencil, if required.

Hard Layer UNIPLATES with inorganic binder are recommended for all applications including those where visualization occurs through strong charring procedures or in any case in which reagent interaction with an organic binder occurs.