Miles Scientific Analtech HPTLC-RPSF (Hydrocarbon Impregnated Silica Reversed Phase, Fluorescent indicator) TLC Plates, Glass, 150 um, 20 x 20 cm, 25/Pk - P55017

Glass-backed Analytical HPTLC Plates

Miles Scientific Analtech Brand HPTLC-RPSF TLC Plates (with Fluorescent indicator UV254) - Glass-backed - 150µm thickness - 20x20cm - 25 plates per box - 8μm particle, 60Å pore - (≈5% carbon load, Inorganic binder)

Product Number: P55017

Weight: 11.10 LBS

• RPS - Hydrocarbon Impregnated (Silica Gel) Reversed Phase Separation
• F - Fluorescent indicator UV254. (Can be added to any adsorbent.)
• HPTLC - High Performance Thin Layer Chromatography

Reversed Phase HPTLC

The availability of both normal phase and Reversed Phase HPTLC UNIPLATES™ completes HPTLC as a sensible, efficient and cost effective alternative technique for quantitative analysis. These Reversed Phase adsorbents share the features and benefits of silica gel HPTLC plates: high efficiency adsorbents, 150 μm layer thickness, and smooth surface for densitometry. In the High Performance format, Analtech offers several varieties of supports. A hydrocarbon impregnated layer, similar to the analytical RPS UNIPLATE, offers complete aqueous solvent compatibility. Three nonpolar bonded supports in the UNIBOND™ series are available: RP2, RP8, and RP18. These fully silanized silica gels share complete compatibility with all organic solvents. However, due to the extreme hydrophobic nature of the RP18 adsorbent, aqueous wettability is limited to approximately 60% water in the mobile phase. Wettability is correspondingly higher with the slightly more polar shorter chain RP series plates.

Hydrocarbon Impregnated

• Less expensive than bonded phase TLC plates
• Aqueous compatibility for polar samples
• Inorganic binder
• Rapid, inexpensive methods development for HPLC
• Expanded flexibility to a wide range of applications

The RPS impregnated UNIPLATE approximates the adsorption characteristics of the bonded C18 modified silica gels. Therefore, RPS Reversed Phase UNIPLATES are an excellent medium for rapid, inexpensive screening of chromatographic conditions in HPLC method development. Mixtures of water or buffers plus a polar organic modifier (eg., Methanol, acetonitrile, THF, etc.) are easily scouted with RPS UNIPLATES. However, since the hydrocarbon is adsorbed but not chemically anchored to the silica gel, the RPS UNIPLATES cannot tolerate nonpolar organic solvents. Ethyl Acetate or less polar organic solvents will strip the hydrocarbon from the silica gel support.