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Nacalai Tesque COSMOSIL 5Diol-120-II Packed Column, 5 µm, 4.6 mm I.D. x 250 mm - 06888-91

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COSMOSIL Diol-120-II - Diol-300-II

COSMOSIL Diol-120-II and Diol-300-II gel filtration columns are ideal for the size-based separation of proteins and other water soluble polymers. The separation MW range is 5,000-700,000 daltons for proteins and 300-300,000 daltons for water soluble polymers when Diol-120-II and Diol-300-II are used in series.

Material characteristics

Packing material5Diol-120-II5Diol-300-II
Silica gelhigh purity porous spherical silica
Average particle size5 µm
Average pore sizeapprox. 120Åapprox. 300Å
Stationary phasediol group
Object substanceprotein, water soluble polymer
Flow rate0.5-1.0 (ml/min)
End capping treatmentnear-perfect treatment
Selection of pore sizeProtein5,000-100,000 Da10,000-700,000 Da
Water soluble polymer300-30,000 Da500-300,000 Da

Calibration curve of proteins

Condition
ColumnCOSMOSIL 5Diol-II 7.5 mm I.D. x 600 mm
Mobile phase20 mmol/l phosphate buffer(pH7.0)+100 mmol/l Na2SO4
Flow rate1.0 ml/min
Temperature30?oC

  • Model: 06888-91
  • Manufactured by: Nacalai Tesque

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