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Nacalai Tesque COSMOSIL Protein-R Packed Column, 5 µm, 20.0 mm I.D. x 150 mm - 06531-41

Price: £1,674.75


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COSMOSIL® Protein-R

COSMOSIL Protein-R is a reversed phased HPLC column designed specifically for peptide and protein separations. This column provides improved separation, high recovery rate and acid-resistant, which are often problematic to separate using conventional C18-300A (wide pore) and C4-300A (wide pore).

Material characteristics

Packing materialProtein-R
Silica gelhigh purity porous spherical silica
Average particle size5 μm
Average pore sizeapprox. 300A
Specific surface areaapprox. 150 m2/g
Stationary phaseoctadecyl group
Bonding typepolymeric
Main interactionshydrophobic interaction
End capping treatmentnear-perfect treatment
Feature- High recovery rate
- Acid-resistance

Comparison of separation

Protein-R shows sharper peaks for proteins than conventional C18 wide pore columns.

Protein-R
5C18-300A
Condition
Column size4.6 mm I.D. x 150 mmSample
  1. Ribonuclease A 1.0 μg
  2. Cytochrome C 1.0 μg
  3. Lysozyme 1.0 μg
  4. BSA 1.0 μg
  5. Myoglobin 1.0 μg
  6. Ovalbumin 1.0 μg
Mobile phaseA : 0.05%TFA - 20% acetonitrile
B : 0.05%TFA - 60% acetonitrile
B conc. 0 → 35% 60 min linear gradient
Flow rate1.0 ml/min
Temperature30°C
DetectionUV 220 nm

Recovery rate

The figure below shows recovery rates for proteins using different columns. Protein-R shows a higher recovery rate than C4-300 and a much higher recovery rate than C18-300.

Durability

The figure below shows durability against acidic mobile phase of various columns. Protein-R shows a higher acid durability than C4-300.


  • Model: 06531-41
  • Manufactured by: Nacalai Tesque

Additional Images


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