Saccharides are not retained on standard C18 columns because of the low hydrophobicity of compounds. COSMOSIL Sugar-D and NH2-MS are specifically designed for separation of saccharides. COSMOSIL C18-PAQ is recommended for hydrophobic glycosides or saccharide derivatives.
Material characteristics
Packing material | Sugar-D | NH2-MS |
---|
Silica gel | high purity porous spherical silica |
---|
Average particle size | 5 μm |
---|
Average pore size | - | approx. 120A |
---|
Specific surface area | - | approx. 300m2/g |
---|
Stationary phase | - | |
---|
secondary/tertiary amine | aminopropyl |
Bonding type | - | polymeric |
---|
Object substance | monosaccharide, oligosaccharide |
---|
End capping treatment | - | near-perfect treatment |
---|
Carbon content | - | approx. 4% |
---|
Feature | - High durability
- Good quantitative analysis
| - Different selectivity from Sugar-D
|
---|
COSMOSIL Sugar-D
Conventionally aminopropyl bonded stationary phases are used for liquid chromatographic analysis of mono- and oligosaccharides. General shortcomings of the conventional aminopropyl bonded phases are tailing and adsorption of certain saccharides and the general low durability (short active life) of these columns. These problems are addressed and solved by the novel COSMOSIL Sugar-D, resulting in better (sharper) separation and much improved durability.
Durability
Decomposition condition |
---|
Solution | water |
---|
Flow rate | 1 ml/min |
---|
Temperature | room temperature |
---|
Condition |
---|
Column size | 4.6 mm I.D.x250 mm |
---|
Mobile phase | acetonitrile : water = 70 : 30 |
---|
Flow rate | 1 ml/min |
---|
Temperature | 30°C |
---|
Detection | RI |
---|
Sample | Maltose |
---|