Nacalai Tesque COSMOSIL 2.5HILIC Microbore Analytical UHPLC Column, 2.5 µm, 130 Å, 2 x 150 mm, 1/Pk - 11770-51

Cosmosil® 2.5HILIC

Nacalai Tesque COSMOSIL 2.5HILIC Microbore Analytical UHPLC Column, 2.5µm, 130Å, 2mm I.D. x 150mm Length, 1/Pk

Product Number: 11770-51

Ultra-High Performance Column for HILIC Hydrophilic Interaction Analysis

Hydrophilic interaction chromatography is a variation of normal phase chromatography in which a polar stationary phase is used with a mobile phase that contains a high concentration of water-miscible organic solvent and a low concentration of aqueous eluent. The main retention mechanism is the partitioning of the polar analytes between the polar stationary and the non-polar mobile phase. As it is also called "aqueous normal phase," the elution order is similar to that of normal phase and the sample elution is in the order of increasing hydrophilicity. Without using ion-pair reagents, COSMOSIL HILIC retains highly polar analytes that would not be retained in reversed phase chromatography. It also shows a weak anion-exchange mechanism with the positively charged stationary phase; thus acidic compounds are strongly retained.

COSMOSIL HILIC is a column for hydrophilic interaction chromatography with Triazole bonded silica packing material. The hydrophilic interaction chromatography is a variation of normal phase chromatography where a polar stationary phase is used with a mobile phase which contains a high concentration of organic solvent and a low concentration of aqueous eluent. The main retention mechanism is the partitioning of the polar analytes between the polar stationary and the non-polar mobile phase. As it is also called "aqueous normal phase", the elution order is similar to that of normal phase and the sample elution is in the order of increasing hydrophilicity. Without using ion-pair reagent COSMOSIL HILIC retains highly polar analytes that would not be retained in reversed phase chromatography. It also shows a weak anion-exchange mechanism with the positively charged stationary phase, thus acidic compound is strongly retained.

• Ultra-High Performance using 2.5 μm particles
• Can be used with any conventional LC systems
• Alternative selectivity to other HILIC columns
• Triazole bonded stationary phase
• Enhanced hydrophilic interaction
• Excellent separation for organic acids

Column Specifications:

  Additional Column Sizes are available - Please contact our Sales Team with your requirements.

Cosmosil Columns Online Catalogue:

• Nacalai Tesque COSMOSIL HPLC Columns
• Nacalai Tesque COSMOCORE UHPLC Columns
• Nacalai Tesque Reagents, Standards, Solvents